Skip to main content
ARS Home » Southeast Area » Auburn, Alabama » Aquatic Animal Health Research » Research » Publications at this Location » Publication #405096
Research Project: Integrated Research to Improve Aquatic Animal Health in Warmwater Aquaculture

Location: Aquatic Animal Health Research

Title: Global gene expression analyses of channel catfish after being fed diets with inclusion of frass from black soldier fly larvae

Author
item Abernathy, Jason
item Lange, Miles
item Aksoy, Mediha
item Eljack, Rashida
item Beck, Benjamin
item MULIYA SANKAPPA, NITHIN - Orise Fellow

Submitted to: NIH Gene Expression Omnibus (GEO) Database
Publication Type: Database / Dataset
Publication Acceptance Date: 5/6/2023
Publication Date: 1/31/2024
Citation: Abernathy, J.W., Lange, M.D., Aksoy, M., Eljack, R.M., Beck, B.H., Muliya Sankappa, N. 2024. Global gene expression analyses of channel catfish after being fed diets with inclusion of frass from black soldier fly larvae. NIH Gene Expression Omnibus (GEO) Database. GSE231874. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE231874

Interpretive Summary:

Technical Abstract: Frass is the by-product of the larval meal industry and includes larval waste, exoskeleton sheds, and residual feed ingredients. Experimental frass was derived from the larvae of black solder flies (Hermetia illucens) fed dried distillers grains. A 10-week study was conducted to evaluate the effect of dietary levels of frass on the global gene expression of channel catfish, Ictalurus punctatus. Three diets containing 0, 50, and 300 g frass per kg diet were fed to channel catfish (5.24 ± 0.04 g) in quadruplicate aquaria to apparent satiation twice daily. Intestine (n=12 in pools of 3) and liver (n=12 in pools of 3) tissues were taken from fish at the end of the trial and processed for high-throughput Illumina RNA sequencing (RNAseq). Pairwise comparisons identified both up- and down-regulated genes in frass diets compared to no frass controls. Raw sequencing reads and processed gene expression data has been submitted to the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) and is available to the public under the accession number GSE231874.