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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #405208
Research Project: Enhancing Control of Stripe Rusts of Cereal Crops

Location: Wheat Health, Genetics, and Quality Research

Title: Genome-wide QTL mapping for stripe rust resistance in spring wheat line PI 660122 using the wheat 15K SNP array

Author
item YAN, QIONG - Southwest University Of Science And Technology
item TAN, WENJING - Southwest University Of Science And Technology
item TIAN, RAN - Southwest University Of Science And Technology
item ZHENG, XIAOCHEN - Southwest University Of Science And Technology
item FENG, JUNMING - Southwest University Of Science And Technology
item LUO, XIAOQIN - Southwest University Of Science And Technology
item SI, BINFAN - Southwest University Of Science And Technology
item LI, XI - Southwest University Of Science And Technology
item HUANG, KEBING - Southwest University Of Science And Technology
item WANG, MEINAN - Washington State University
item Chen, Xianming
item REN, YONG - Southwest University Of Science And Technology
item YANG, SUIZHUANG - Southwest University Of Science And Technology
item ZHOU, XINLI - Southwest University Of Science And Technology

Submitted to: Frontiers in Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/31/2023
Publication Date: 8/28/2023
Citation: Yan, Q., Tan, W., Tian, R., Zheng, X., Feng, J., Luo, X., Si, B., Li, X., Huang, K., Wang, M., Chen, X., Ren, Y., Yang, S., Zhou, X. 2023. Genome-wide QTL mapping for stripe rust resistance in spring wheat line PI 660122 using the wheat 15K SNP array. Frontiers in Plant Science. 14:1232897. https://doi.org/10.3389/fpls.2023.1232897.
DOI: https://doi.org/10.3389/fpls.2023.1232897

Interpretive Summary: Stripe rust is a global disease of wheat. Identification of new resistance genes is key to develop and grow resistant varieties for control of the disease. Wheat line PI 660122 has exhibited a high level of stripe rust resistance for over a decade. However, the genetics of stripe rust resistance in this line has not been studied. A set of 239 recombinant inbred lines (RILs) was developed from a cross between PI 660122 and an elite Chinese cultivar Zhengmai 9023. The RIL population was phenotyped for stripe rust response in three field environments and genotyped with the Wheat 15K SNP (single nucleotide polymorphism) array. A total of 9 quantitative trait loci (QTL) for stripe rust resistance were mapped to chromosomes 1B (1 QTL), 2B (1), 4B (2 QTL), 4D (2), 6A (1), 6D (1), and 7D (1), of which 7 QTL were stable. One of the two QTL on 4D was a major all-stage resistance QTL explaining the highest percentage (10.67–20.97%) of the total phenotypic variation. These QTL and their linked SNP markers in this study can be used in wheat breeding to improve resistance to stripe rust. In addition, 26 lines were selected based on stripe rust resistance and agronomic traits in the field for further selection and release of new cultivars.

Technical Abstract: Stripe rust is a global disease of wheat. Identification of new resistance genes is key to develop and grow resistant varieties for control of the disease. Wheat line PI 660122 has exhibited a high level of stripe rust resistance for over a decade. However, the genetics of stripe rust resistance in this line has not been studied. A set of 239 recombinant inbred lines (RILs) was developed from a cross between PI 660122 and an elite Chinese cultivar Zhengmai 9023. The RIL population was phenotyped for stripe rust response in three field environments and genotyped with the Wheat 15K SNP (single nucleotide polymorphism) array. A total of 9 quantitative trait loci (QTL) for stripe rust resistance were mapped to chromosomes 1B (1 QTL), 2B (1), 4B (2 QTL), 4D (2), 6A (1), 6D (1), and 7D (1), of which 7 QTL were stable and designated QYrPI660122.swust-4BS, QYrPI660122.swust-4BL, QYrPI660122.swust-4DS, QYrPI660122.swust-4DL, QYrZM9023.swust-6AS, QYrZM9023.swust-6DS, and QYrPI660122.swust-7DS. QYrPI660122.swust-4DS was a major all-stage resistance QTL explaining the highest percentage (10.67–20.97%) of the total phenotypic variation and was mapped to a 12.15-cM interval flanked by SNP markers AX-110046962 and AX-111093894 on chromosome 4DS. The QTL and their linked SNP markers in this study can be used in wheat breeding to improve resistance to stripe rust. In addition, 26 lines were selected based on stripe rust resistance and agronomic traits in the field for further selection and release of new cultivars.