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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #405784
Research Project: Discovery and Improvement of Traits to Enhance Sorghum as a Multiple Purpose Crop

Location: Plant Stress and Germplasm Development Research

Title: A large Sequenced Mutant library covers 98% of the genes in the genome of the inbred line BTx623

Author
item Xin, Zhanguo
item JIAO, YINPING - Texas Tech University
item Burow, Gloria
item Hayes, Chad
item Chen, Junping
item Pugh, Nicholas - Ace
item Ware, Doreen

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/12/2023
Publication Date: 10/29/2023
Citation: Xin, Z., Jiao, Y., Burow, G.B., Hayes, C.M., Chen, J., Pugh, N.A., Ware, D. 2023. A large Sequenced Mutant library covers 98% of the genes in the genome of the inbred line BTx623. ASA-CSSA-SSSA Annual Meeting Abstracts.

Interpretive Summary:

Technical Abstract: Induced mutagenesis is a powerful approach to generate variations for elucidation of gene function and create new traits for breeding. We have developed a pedigreed mutant library through chemical mutagenesis with ethylmethane sulfonate (EMS) treated seeds in sorghum and sequenced 1256 randomly selected lines. The mutant library displayed many traits of significant agronomic value. This endeavor resulted in detection and cataloguing of over 10 million canonical EMS-induced mutations (or variants), covering over 98% of the genes in the genome of inbred line BTx623. Additionally, 610,320 mutations were identified in the promoter and enhancer regions of 18,000 and 11,790 genes, respectively. Mutations in genes of interest can be searched online through SorghumBase (https://www.sorghumbase.org) or SorbMuDB (https://www.depts.ttu.edu/igcast/SorbMutDB.php ). Our sequence-indexed sorghum mutant population provides an excellent platform to discover gene functions in the grass family, supporting plant biology research and crop breeding.