Periconceptual maternal nutrition affects fetal liver programming of energy- and lipid-related genes

Authors: DINIZ, W - Auburn University; WARD, A - North Dakota State University; MCCARTHY, K - University Of Nebraska; KASSETAS, C - North Dakota State University; BAUMGAERTNER, F - North Dakota State University; REYNOLDS, L - North Dakota State University; BOROWICZ, P - North Dakota State University; SEDIVIC, K - North Dakota State University; KIRSCH, J - North Dakota State University; DORSAM, S - North Dakota State University; NEVILLE, T - North Dakota State University; FORCHERIO, J - Purina; SCOTT, R - Purina; CATON, J - North Dakota State University; DAHLEN, C - North Dakota State University

Submitted to: Animals
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/7/2023
Publication Date: 2/9/2023
Citation: Diniz, W.J., Ward, A.K., Mccarthy, K.L., Kassetas, C.J., Baumgaertner, F., Reynolds, L.P., Borowicz, P.P., Sedivic, K.K., Kirsch, J.D., Dorsam, S.T., Neville, T.L., Forcherio, J.C., Scott, R., Caton, J.S., Dahlen, C.R. 2023. Periconceptual maternal nutrition affects fetal liver programming of energy- and lipid-related genes. Animals. 13(4):600. https://doi.org/10.3390/ani13040600.
DOI: https://doi.org/10.3390/ani13040600

Interpretive Summary: Maternal nutrient supply during the periconceptual period has long-term effects on fetal development and tissue function. During pregnancy, the fetus relies on the dam for its nutrient supply. Our objective was to ascertain whether the periconceptual and early life plan of nutrition could affect fetal liver development and gene expression profiles. To this end, we investigated the impacts of maternal vitamin and mineral supplementation (from pre-breeding to day 83) and two rates of body-weight gain during the first 83 days of pregnancy in female fetuses of crossbred Angus beef heifers. We identified 591 unique differentially expressed genes across all six vitamin-gain contrasts. Over-represented pathways were related to energy metabolism, lipid metabolism, and mineral and amino acid transport. Our findings suggest that periconceptual maternal nutrition affects fetal hepatic function through altered expression of energy- and lipid-related genes.

Technical Abstract: During pregnancy, the fetus relies on the dam for its nutrient supply. Nutritional stimuli during fetal organ development can program hepatic metabolism and function. Herein, we investigated the role of vitamin and mineral supplementation (VTM or NoVTM—at least 71 days pre-breeding to day 83 of gestation) and rate of weight gain (low (LG) or moderate (MG)—from breeding to day 83) on the fetal liver transcriptome and the underlying biological pathways. Crossbred Angus beef heifers (n = 35) were randomly assigned to one of four treatments in a 2 2 factorial design (VTM_LG, VTM_MG, NoVTM_LG, and NoVTM_MG). Gene expression was measured with RNA-Seq in fetal livers collected on day 83 0.27 of gestation. Our results show that vitamin and mineral supplementation and rate of weight gain led to the differential expression of hepatic genes in all treatments. We identified 591 unique differentially expressed genes across all six VTM-gain contrasts (FDR 0.1). Over-represented pathways were related to energy metabolism, including PPAR and PI3K-Akt signaling pathways, as well as lipid metabolism, mineral transport, and amino acid transport. Our findings suggest that periconceptual maternal nutrition affects fetal hepatic function through altered expression of energy- and lipid-related genes.