Dataset of RNA-Seq transcriptome of the fetal liver at day 83 of gestation associated with periconceptual maternal nutrition in beef heifers

Authors: DINIZ, W - Auburn University; WARD, A - North Dakota State University; MCCARTHY, K - University Of Nebraska; KASSETAS, C - North Dakota State University; BAUMGAERTNER, F - North Dakota State University; REYNOLDS, L - North Dakota State University; BOROWICZ, P - North Dakota State University; SEDIVIC, K - North Dakota State University; KIRSCH, J - North Dakota State University; DORSAM, S - North Dakota State University; NEVILLE, T - North Dakota State University; FORCHERIO, J - Purina; SCOTT, R - Purina; CATON, J - North Dakota State University; DAHLEN, C - North Dakota State University

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/17/2023
Publication Date: 4/23/2023
Citation: Diniz, W.J., Ward, A.K., Mccarthy, K.L., Kassetas, C.J., Baumgaertner, F., Reynolds, L.P., Borowicz, P.P., Sedivic, K.K., Kirsch, J.D., Dorsam, S.T., Neville, T.L., Forcherio, J.C., Scott, R., Caton, J.S., Dahlen, C.R. 2023. Dataset of RNA-Seq transcriptome of the fetal liver at day 83 of gestation associated with periconceptual maternal nutrition in beef heifers. Data in Brief. 48:109173. https://doi.org/10.1016/j.dib.2023.109173.
DOI: https://doi.org/10.1016/j.dib.2023.109173

Interpretive Summary: Publication presents a dataset based on the RNA-Seq analysis of liver tissue from bovine female fetuses at day 83 of gestation. These data were generated to investigate the effects of periconceptual maternal vitamin and mineral supplementation and rates of body weight gain on the transcript abundance of genes associated with fetal hepatic metabolism and function. To our knowledge, this is the first dataset investigating the fetal liver transcriptome in response to periconceptual maternal vitamin and mineral supplementation and/or the rate of weight gain. The data de- scribed in this article provides genes and molecular pathways differentially programming liver development and function.

Technical Abstract: Presents a dataset based on the RNA-Seq analysis of liver tissue from bovine female fetuses at day 83 of gestation. The findings were reported in the main article, “Periconceptual maternal nutrition affects fetal liver programming of energy- and lipid-related genes”. These data were generated to investigate the effects of periconceptual maternal vitamin and mineral supplementation and rates of body weight gain on the transcript abundance of genes associated with fetal hepatic metabolism and function. To this end, crossbred Angus beef heifers (n = 35) were randomly assigned to 1 of 4 treatments in a 2 ×2 factorial design. The main effects tested were vitamin and mineral supplementation (VTM or NoVTM –at least 71 days pre-breeding to day 83 of gestation) and rate of weight gain (low (LG –0.28 kg/d) or moderate (MG –0.79 kg/d) –from breeding to day 83). The fetal liver was collected on day 83 ±0.27 of gestation. After total RNA isolation and quality control, strand-specific RNA libraries were prepared and sequenced on the Illumina®NovaSeq 60 0 0 platform to generate paired-end 150-bp reads. After read mapping and counting, differential expression analysis was performed with edgeR. We identified 591 unique differentially expressed genes across all six vitamin- gain contrasts (FDR =0.1). To our knowledge, this is the first dataset investigating the fetal liver transcriptome in response to periconceptual maternal vitamin and mineral sup- plementation and/or the rate of weight gain. The data described in this article provides genes and molecular pathways differentially programming liver development and function.