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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Mycology and Nematology Genetic Diversity and Biology Laboratory » Research » Publications at this Location » Publication #407853
Research Project: Fungal Systematics and Diagnostic Resource Development for Safeguarding Plant Health

Location: Mycology and Nematology Genetic Diversity and Biology Laboratory

Title: Fluorescence in situ hybridization protocol for visualization of oomycetes in vitro and in planta

Author
item GROBEN, GLEN - Oak Ridge Institute For Science And Education (ORISE)
item Salgado-Salazar, Catalina
item Crouch, Joanne

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 9/29/2023
Publication Date: 12/31/2024
Citation: Groben, G., Salgado-Salazar, C., Crouch, J. 2024. Fluorescence in situ hybridization protocol for visualization of oomycetes in vitro and in planta. Methods in Molecular Biology. 2892. https://doi.org/10.1007/978-1-0716-4330-3.
DOI: https://doi.org/10.1007/978-1-0716-4330-3

Interpretive Summary: Species in the oomycetes are among the most destructive plant pathogens worldwide, affecting many crops important for the survival of humans and animals. Visualization of pathogens within the affected host plants is one of the first steps in pathogen identification. Fluorescent tags are a way to visualize pathogens in plants and can be used to study other pathogen interactions. This information will be used by extension personnel, plant breeders, plant pathologists and others engaged in the biology and management of plant diseases.

Technical Abstract: Fluorescence in situ hybridization enables the visualization of organisms in the environment without having to culture them. Here we describe a FISH protocol to visual oomycete structures (mycelium, sporangiophores, oospores) directly and from colonized plant material. The protocol utilizes organic compounds with low toxicities and does not require a permeabilization step. The protocol was used to visualize impatiens downy mildew Plasmopara destructor sporangiophores colonizing an Impatiens walleriana leaf.