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ARS Home » Plains Area » El Reno, Oklahoma » Oklahoma and Central Plains Agricultural Research Center » Peanut and Small Grains Research Unit » Research » Publications at this Location » Publication #408213
Research Project: Genetic Improvement for Resistance to Aphids and Major Diseases in Wheat, Barley, and Sorghum in the Great Plains

Location: Peanut and Small Grains Research Unit

Title: Characterization of quantitative trait loci for leaf rust resistance in the Uzbekistani wheat landrace Teremai Bugdai

Author
item Xu, Xiangyang
item Li, Genqiao
item Bai, Guihua
item BIAN, RUOLIN - Kansas State University
item Bernardo, Amy
item Kolmer, James
item CARVER, BRETT - Oklahoma State University
item WATIRA, TEZERA WOLABU - Oklahoma State University
item WU, YANQI - Oklahoma State University

Submitted to: Phytopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/5/2024
Publication Date: 5/28/2024
Citation: Xu, X., Li, G., Bai, G., Bian, R., Bernardo, A.E., Kolmer, J.A., Carver, B.F., Watira, T., Wu, Y. 2024. Characterization of quantitative trait loci for leaf rust resistance in the Uzbekistani wheat landrace Teremai Bugdai. Phytopathology. 114:1373-1379. https://doi.org/10.1094/PHYTO-09-23-0320-R.
DOI: https://doi.org/10.1094/PHYTO-09-23-0320-R

Interpretive Summary: Leaf rust, caused by Puccinia triticina Eriks. (Pt), is a major cause of wheat yield losses globally, and novel leaf rust resistance genes are needed to enhance wheat leaf rust resistance in the USA. Teremai Bugdai is an Uzbekistani landrace that exhibits resistance to many U.S. Pt races. To unravel leaf rust resistance loci in Teremai Bugdai, a recombinant inbred line (RIL) population of Teremai Bugdai × TAM 110 was evaluated for response to Pt race Pt54-1 and genotyped using single nucleotide polymorphism (SNP) markers generated by genotyping-by-sequencing. Genetic analysis indicated that three leaf rust resistance loci on the short arm of chromosome 2D, the long arm of chromosome 6B, and the long arm of chromosome 7B conditioned leaf rust resistance in Teremai Bugdai. Nine user-friendly, high throughput KASP markers were developed to facilitate the introgression of the three leaf rust resistance loci into locally adapted wheat lines.

Technical Abstract: Leaf rust, caused by Puccinia triticina Eriks. (Pt), is a major cause of wheat yield losses globally, and novel leaf rust resistance genes are needed to enhance wheat leaf rust resistance. Teremai Bugdai is a Uzbekistani landrace that exhibits resistance to many U.S. Pt races. To unravel leaf rust resistance loci in Teremai Bugdai, a recombinant inbred line (RIL) population of Teremai Bugdai × TAM 110 was evaluated for response to Pt race Pt54-1 (TNBGJ) and genotyped using single nucleotide polymorphism (SNP) markers generated by genotyping-by-sequencing (GBS). QTL analysis using 5,130 high quality GBS-SNPs revealed three QTLs, QLr-Stars-2DS, QLr-Stars-6BL, and QLr.stars-7BL, for leaf rust resistance in two experiments. QLr-Stars-2DS was delimited to a ~ 19.47 Mb interval between 46.4 Mb and 65.9 Mb on the short arm of chromosome 2D and explained 31.3% and 33.2% of the phenotypic variance in the two experiments. QLr-Stars-6BL was mapped in a ~84.0 Kb interval between 719.48 Mb and 719.56 Mb on the long arm of chromosome 6B, accounting for 33% to 36.8% of the phenotypic variance in two experiments. QLr.stars-7BL was mapped to a 350 Kb interval between 762.41 Mb and 762.76 Mb on the long arm of chromosome 7B and explained 4.4% to 5.3% of the phenotypic variance. Nine GBS-SNPs flanking these QTLs were converted to KASP markers and these markers can be used to facilitate their introgression into locally adapted wheat lines.