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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #408375
Research Project: Virulence Mechanisms, Microbiome Changes and Control Strategies for Priority Bacterial Infections in Swine

Location: Virus and Prion Research

Title: The contribution of BvgR, RisA, and RisS to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation in bordetella bronchiseptica

Author
item Nicholson, Tracy
item WAACK, URSULA - Oak Ridge Institute For Science And Education (ORISE)
item Fleming, Damarius
item CHEN, QING - Us Biologics
item MILLER, LAURA - Kansas State University
item MERKEL, TOD - Us Biologics
item STIBITZ, SCOTT - Us Biologics

Submitted to: Frontiers in Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/20/2023
Publication Date: 3/7/2024
Citation: Nicholson, T.L., Waack, U., Fleming, D.S., Chen, Q., Miller, L.C., Merkel, T.J., Stibitz, S. 2024. The contribution of BvgR, RisA, and RisS to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation in bordetella bronchiseptica . Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2024.1305097.
DOI: https://doi.org/10.3389/fmicb.2024.1305097

Interpretive Summary: Bordetella bronchiseptica is pervasive in swine populations and plays multiple roles in respiratory disease. This report investigated three proteins known to regulate the productions of factors that allow this pathogen to transmit and cause disease. All the factors regulated by the three proteins were identified. Targeting these newly identified factors for future vaccines will not only decrease disease caused by Bordetella but also decrease host-to-host transmission as well, subsequently reducing infections and secondary bacterial infections in swine.

Technical Abstract: Bordetella bronchiseptica is a highly contagious respiratory bacterial veterinary pathogen. In this study the contribution of the transcriptional regulators BvgR, RisA, RisS, and the phosphorylation of RisA to global gene regulation, intracellular cyclic-di-GMP levels, motility, and biofilm formation were evaluated. RNA-sequencing was used to evaluate the global gene regulation resulting in the identification of both virulence-activated and virulence-repressed genes that were and were not regulated by each of these factors. The BvgAS system, along with BvgR, RisA, and the phosphorylation of RisA served a role in in c-di-GMP degradation. BvgR and unphosphorylated RisA were found to temporally regulate motility. Additionally, BvgR, RisA, and RisS were found to be required for biofilm formation.