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ARS Home » Northeast Area » Newark, Delaware » Beneficial Insects Introduction Research Unit » Research » Publications at this Location » Publication #408858
Research Project: Genetics and Genomics of Introduced Species for Biological Control of Invasive Species

Location: Beneficial Insects Introduction Research Unit

Title: Reduced-representation libraries in insect genomics

Author
item Hopper, Keith

Submitted to: Current Opinion in Insect Science
Publication Type: Literature Review
Publication Acceptance Date: 5/8/2023
Publication Date: 7/11/2023
Citation: Hopper, K.R. 2023. Reduced-representation libraries in insect genomics. Current Opinion in Insect Science. https://doi.org/10.1016/j.cois.2023.101084.
DOI: https://doi.org/10.1016/j.cois.2023.101084

Interpretive Summary: Sequencing of random parts of genomes can now be done for any species. Here I review research on this topic during the last two years, report meta-analysis of the results, and discuss analysis methods and issues. Scanning the literature from 2021-2022 identified 21 papers, the majority of which were on population differences, including local adaptation and migration, but several papers were on genetic maps and their use in assembly scaffolding or analysis adaptations, on the origin of incursions of pest insects, or on infection rates of a pathogen in a disease vector. The research reviewed covers 24 species from 18 families and 10 orders. Analysis showed that less than 10 percent, and most often, less than 1 percent of the genome was implicated in local adaptation and that the number of adaptive loci correlated with divergence among populations.

Technical Abstract: Genotyping-by-sequencing of reduced-representation libraries has ushered in an era where genome-wide data can be gotten for any species. Here I review research on this topic during the last two years, report meta-analysis of the results, and discuss analysis methods and issues. Scanning the literature from 2021-2022 identified 21 papers, the majority of which were on population differences, including local adaptation and migration, but several papers were on genetic maps and their use in assembly scaffolding or analysis of quantitative trait loci, on the origin of incursions of pest insects, or on infection rates of a pathogen in a disease vector. The research reviewed covers 24 species from 18 families and 10 orders. Meta-analysis showed that less than 10 percent, and most often, less than 1 percent of the genome was implicated in local adaptation and that the number of adaptive loci correlated with genetic divergence among populations.