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Research Project: Development of Novel Cottonseed Products and Processes

Location: Commodity Utilization Research

Title: Lipopolysaccharide regulation of antiinflammatory tristetraprolin family and proinflammatory cytokine gene expression in mouse macrophages

Author
item Cao, Heping

Submitted to: BMC Research Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/8/2024
Publication Date: 3/19/2024
Citation: Cao, H. 2024. Lipopolysaccharide regulation of antiinflammatory tristetraprolin family and proinflammatory cytokine gene expression in mouse macrophages. BMC Research Notes. 17. Article 82. https://doi.org/10.1186/s13104-024-06743-6.
DOI: https://doi.org/10.1186/s13104-024-06743-6

Interpretive Summary: Tristetraprolin (TTP/ZFP36) family proteins exhibit antiinflammatory effects by destabilizing proinflammatory cytokine mRNAs. We showed previously that bacterial endotoxin lipopolysaccharides (LPS) stimulate TTP and tumor necrosis factor (TNF) gene expression, but less is known about LPS effects on TTP homologies and other proinflammatory cytokine gene expression in macrophages. The objective was to investigate LPS regulation of TTP family gene and TTP-targeted cytokine gene expression in mouse RAW264.7 macrophages. Results showed that LPS had minimal effects on cell viability and soluble protein content in the cells but dramatically increased antiinflammatory TTP gene expression as well as proinflammatory TNF and COX2 gene expression in the mouse macrophages.

Technical Abstract: Objective: Tristetraprolin (TTP/ZFP36) family proteins exhibit antiinflammatory effects by destabilizing proinflammatory cytokine mRNAs. Previous study showed that bacterial endotoxin lipopolysaccharides (LPS) stimulate TTP and tumor necrosis factor (TNF) gene expression, but less is known about LPS effects on TTP homologues and other proinflammatory cytokine gene expression in macrophages. The objective was to investigate LPS regulation of TTP family gene and TTP-targeted cytokine gene expression in mouse RAW264.7 macrophages. Results: MTT assay showed that cell viability was not significantly affected by LPS treatment up to 1000 ng/mL for 2-24h. LPS only mildly affected the soluble protein content in the cells. qPCR assay showed that LPS stimulated TTP mRNA rapidly but not sustainable with 40, 10, and 3 fold of control after 2, 8 and 24 h treatment, respectively. LPS decreased ZFP36L2 mRNA by 20-30% and exhibited minimal effects on ZFP36L1 and ZFP36L3 mRNA levels. LPS increased mRNA levels of TNF, COX2, GM-CSF, INF' and IL12b up to 311, 418, 11, 9 and 4 fold, respectively. This study demonstrated that LPS dramatically increased antiinflammatory TTP gene expression as well as proinflammatory TNF and COX2 gene expression but had only mild effects on TTP homologues and other proinflammatory cytokine gene expression in the mouse macrophages.