Indomethacin and 20-Hydroxyecdysome influence protein expression in a Spodoptera frugiperda nervous system cell line

Authors: WANG, YONG - Shenyang Agricultural University; BUER, BENJAMIN - Bayer Crop Sciences, Germany; Goodman, Cynthia; Kang, Dave; REALL, TAMRA - University Of Missouri; DOHN, SUSANNE - Bayer Crop Sciences, Germany; Ringbauer, Joseph - Joe; LI, YAOFA - Hebei Academy Of Agriculture; GEIBEL, SVEN - Bayer Crop Sciences, Germany; Stanley, David

Submitted to: Archives of Insect Biochemistry and Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/23/2024
Publication Date: 2/26/2024
Citation: Wang, Y., Buer, B., Goodman, C.L., Kang, D.S., Reall, T., Dohn, S., Ringbauer Jr, J.A., Li, Y., Geibel, S., Stanley, D.W. 2024. Indomethacin and 20-Hydroxyecdysome influence protein expression in a Spodoptera frugiperda nervous system cell line. Archives of Insect Biochemistry and Physiology. https://doi.org/10.1002/arch.22089.
DOI: https://doi.org/10.1002/arch.22089

Interpretive Summary: There are over 1 million species of insects, most of which are harmless or beneficial to humans. A relatively small number of insects species are responsible for tremendous crop losses, to the extent of famines, at the global level. Scientists working in government, industrial and university laboratories around the world are working to develop highly improved insecticidal chemicals that are safe and healthy to the environment while protecting global food production. In this paper we report on the influence of insect signaling molecules on an established insect cell line. We found that altering the signaling molecules leads to changes in expression of some proteins. Because proteins are active components of cells, understanding protein expression provides new insights into the functions of insect cells. This new information will be used by scientists at the global level to develop advanced insecticides that will ensure production of healthy, safe and attractive foods for a growing global population.

Technical Abstract: Insecticide mode of action studies provide insights into how new insecticidal actives function and contribute to assessing safety to humans and non-target organisms. Insect cell lines that express potential target sites can serve as valuable tools in this effort. We report on the influence of two signaling molecules on protein expression in a nervous system cell line established from Spodoptera frugiperda (Bayer/BCIRL-SfNS2-0714-TR). Cells were exposed to the insect developmental hormone (1 µg/mL 20-hydroxyecdysone, 20E) and/or a cyclooxygenase inhibitor (25 'M indomethacin, INDO) for 24 hr (Day 2), 72 hr (Day 4), or 120 hr (Day 6). Changes in expression levels and their related protein identities were determined using LC-MS + MS-MS. The largest number of changes in protein expression occurred on Day 2. The combination of 20E and INDO led to 222 differentially expressed proteins; 20E and, separately, INDO led to changes in 30 proteins each (p-value < 0.01; >2X or <0.5X-fold changes). We recorded changes in expression of 9 or 12 proteins (20E), 10 or 6 proteins (INDO), and 21 or 20 proteins (20E+INDO) on D4 and D6, respectively. In spite of the cell lines origin form neuronal tissue, the differentially expressed proteins were involved in a variety of fundamental processes beyond neuronal function, with GO term analysis and enrichment providing an in-depth understanding of the influence of these treatments on the SfNS2 cells., changes in their expression levels due to these signaling molecules provide insights into alterations in cell functions Because proteins are active components of cell physiology in their role as enzymes, receptors, elements of signaling transduction pathways and cellular structures, changes in their expression levels under the influence of signaling molecules provide deep insights into their function in cell physiology.