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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #409422
Research Project: Improving Soybean Seed Composition, Plant Productivity, and Resilience to Climate Change Through Biological Network Modification

Location: Plant Genetics Research

Title: Isotopically nonstationary metabolic flux analysis of plants: recent progress and future opportunities

Author
item KOLEY, SOMNATH - Donald Danforth Plant Science Center
item JYOTI, POONAM - Donald Danforth Plant Science Center
item LINGWAN, MANEESH - Donald Danforth Plant Science Center
item Allen, Douglas - Doug

Submitted to: New Phytologist
Publication Type: Review Article
Publication Acceptance Date: 3/6/2024
Publication Date: 4/16/2024
Citation: Koley, S., Jyoti, P., Lingwan, M., Allen, D.K. 2024. Isotopically nonstationary metabolic flux analysis of plants: recent progress and future opportunities. New Phytologist. 242(5):1977-1918. https://doi.org/10.1111/nph.19734.
DOI: https://doi.org/10.1111/nph.19734

Interpretive Summary: Not required for review articles.

Technical Abstract: Metabolic flux analysis (MFA) is a valuable tool for quantifying cellular phenotypes and to guide plant metabolic engineering. By introducing stable isotopic tracers and employing mathematical models, MFA can quantify the rates of metabolic reactions through biochemical pathways. Recent applications of isotopically nonstationary MFA (INST-MFA) to plants have elucidated nonintuitive metabolism in leaves under optimal and stress conditions, described coupled fluxes for fast-growing algae, and produced a synergistic multi-organ flux map that is a first in MFA for any biological system. These insights could not be elucidated through other approaches and showthe potential ofINST-MFA to correct an oversimplified understanding of plant metabolism.