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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Publications at this Location » Publication #410168
Research Project: Surveillance, Pathogen Biology, and Host Resistance of Cereal Rusts

Location: Cereal Disease Lab

Title: High-resolution genetic mapping and identification of candidate genes for the wheat stem rust resistance gene Sr8155B1

Author
item WANG, JIAN - Peking University
item LI, HONGYU - Peking University
item SHEN, TAO - Peking University
item LYU, SHIKAI - Peking University
item REHMAN, SHAMS - Peking University
item LI, HONGNA - Peking University
item WANG, GUIPING - Peking University
item XU, BINYANG - Peking University
item WANG, QING - Peking University
item HU, WANYI - Peking University
item LI, KAIRONG - Peking University
item BAI, SHENGSHENG - Peking University
item MA, JIAN - Sichuan Agricultural University
item YU, HAITAO - Weifang University Of Science And Technology
item Rouse, Matthew
item CHEN, SHISHENG - Peking University

Submitted to: The Crop Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/24/2023
Publication Date: N/A
Citation: N/A

Interpretive Summary: Wheat stem rust is a devastating fungal disease. New virulent strains of the wheat stem rust pathogen threaten to global wheat production. We identified molecular markers closely linked to a wheat stem rust resistance gene that is effective to select virulent strains of the stem rust pathogen. The molecular markers identified can be used to help wheat breeders develop resistant wheat cultivars to protect US and global wheat from threatening virulent strains of the stem rust pathogen.

Technical Abstract: Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), threatens global wheat production. Development of cultivars with increased resistance to stem rust by identification, mapping, and deployment of resistance genes is the best strategy for controlling the disease. In this study, we performed fine mapping and characterization of the all-stage stem rust resistance (Sr) gene Sr8155B1 from the durum wheat line 8155-B1. In seedling tests of biparental populations, Sr8155B1 was effective against six Chinese Pgt races tested. In a segregating population of 5060 gametes, Sr8155B1was mapped to a 0.06-cM region flanked by markersPku2772 and Pku43365, corresponding to 1.5- and 2.7-Mb regions in the Svevo and Chinese Spring reference genomes. Both regions include several typical nucleotide-binding leucine-rich repeat (NLR) and protein kinase genes that represent candidate genes. Among them, three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line TdS4. Four markers (Pku2772,Pku43365,Pku2950, and Pku3721) developed in this study, together with seedling resistance responses, correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes tested. The presence of Sr8155B1in tetraploid wheat accessions CItr 14916, PI 197492, and PI 197493 was confirmed by mapping in three F2 populations. The genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs.