Location: Plant Genetics Research
Title: Impaired skeletal development by disruption of presenilin-1 in pigs and generation of novel pig models for Alzheimer’s diseaseAuthor
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UH, KYUNGJUN - Korea Research Institute Of Bioscience And Biotechnology |
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MONARCH, KAYLYNN - University Of Missouri |
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REESE, EMILY - University Of Missouri |
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RODRIGUEZ, KATHERINE - University Of Missouri |
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YOON, JUNCHUL - University Of Missouri |
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SPATE, LEE - University Of Missouri |
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SAMUEL, MELISSA - University Of Missouri |
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KOH, SEHWON - University Of Missouri |
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Chen, Paula |
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JAROME, TIMOTHY - Virginia Polytechnic Institution & State University |
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ALLEN, TIMOTHY - Florida International University |
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PRATHER, RANDALL - University Of Missouri |
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LEE, KIHO - University Of Missouri |
Submitted to: Journal of Alzheimer's Disease
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 6/27/2024 Publication Date: N/A Citation: N/A Interpretive Summary: Several genes have been linked to early onset of Alzheimer’s disease with one of them being presenilin 1 (PSEN1). The role of PSEN1 was investigated in pigs which have been shown to be suitable models of disease in humans. After introducing mutations in PSEN1, pigs were born but died shortly after birth due to severe skeletal system defects that primarily impacted the spine. Additionally, pigs lacking function PSEN1 had abnormal distribution of different cell populations within the brain that could be linked to the development of neurodegenerative disorders, such as Alzheimer's disease. These pigs will serve as a novel resource to study pathogenesis of different diseases. Technical Abstract: Presenilin 1 (PSEN1) is one of the genes linked to the prevalence of the early onset Alzheimer’s disease. Missense mutations in PSEN1 that alter the function of '-secretase lead to abnormal processing of the amyloid precursor protein (APP) and subsequent accumulation of pathogenic amyloid peptides. In mice, inactivation of Psen1 leads to developmental defects, including vertebral malformation and neural development, ultimately resulting in a perinatal lethality. However, little is known on the role of PSEN1 on the development in other species. To investigate the role of PSEN1 in vertebral development and the pathogenic mechanism of neurodegeneration, by using the CRISPR/Cas9 system we generated pigs carrying different mutations flanking exon 9 including ones with a deleted exon 9 ('exon9), which is a causative mutation for early onset Alzheimer’s disease. Pigs with a PSEN1 null mutation ('exon9-12) died shortly after birth and had significant axial skeletal defects, in agreement with the mouse studies, whereas pigs carrying at least one 'exon9 allele developed normally and remained healthy. Effects of the null mutation on the impaired skeletal development was also observed in fetuses at day 40 of gestation. The lack of functional PSEN1 also triggered an abnormal distribution of astrocytes and microglia in their brain. Founder pigs carrying different mutations on PSEN1 were bred to establish PSEN1'E9/+ to study their relevance to clinical Alzheimer’s diseases. The pigs will serve as a novel resource to study pathogenesis of the diseases. |