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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #410462
Research Project: Host-pathogen Interactions and Control Strategies for Bacterial Respiratory Pathogens in Cattle

Location: Ruminant Diseases and Immunology Research

Title: Analysis of nonsynonymous SNPs in candidate genes that influence bovine temperament and evaluation of their effect in Brahman cattle

Author
item RUIZ-DE LA CRUZ, GILBERTO - Instituto Politécnico Nacional, Centro De Desarrollo De Productos Bioticos (CEPROBI)
item SIFUENTES-RINCON, ANA - Instituto Politécnico Nacional, Centro De Desarrollo De Productos Bioticos (CEPROBI)
item PAREDES-SANCHEZ, FRANCISCO - University Of Tamaulipas
item PARRA-BRACAMONTES, GASPAR - Instituto Politécnico Nacional, Centro De Desarrollo De Productos Bioticos (CEPROBI)
item Casas, Eduardo
item RILEY, DAVID - Texas A&M University
item PERRY, GEORGE - Texas A&M Agrilife
item WELSH JR., THOMAS - Texas A&M University
item RANDEL, RONALD - Texas A&M Agrilife

Submitted to: Molecular Biology Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/17/2024
Publication Date: 2/7/2024
Citation: Ruiz-De La Cruz, G., Sifuentes-Rincon, A.M., Paredes-Sanchez, F.A., Parra-Bracamontes, G.M., Casas, E., Riley, D.G., Perry, G.A., Welsh Jr., T.H., Randel, R.D. 2024. Analysis of nonsynonymous SNPs in candidate genes that influence bovine temperament and evaluation of their effect in Brahman cattle. Molecular Biology Reports. 51(1). Article 285. https://doi.org/10.1007/s11033-024-09264-4.
DOI: https://doi.org/10.1007/s11033-024-09264-4

Interpretive Summary: Cattle temperament is an economically relevant trait in cattle. Brahman is known to be temperamental when compared to European breeds. Nonsynonymous single nucleotide polymorphisms (nsSNP) are variants in the coding region of the genes where the amino acid is altered in the protein. The objective was to identify nsSNP in candidate genes known to be associated with cattle temperament. Three nsSNP were identified in two genes and evaluated their association with temperament in an independent cattle population. Only one nsSNP in the HTR1B gene was associated with cattle temperament. This gene has been associated with mental disorders in humans, behavior in humans and dogs. It is a suitable single nucleotide polymorphism in a candidate gene to explain differences in behavior in cattle.

Technical Abstract: Three nonsynonymous SNPs (nsSNPs) identified in HTR1B and SLC18A2 candidate genes for temperament were analyzed in silico to determine their effects on protein structure. Those nsSNPs allowing changes were selected for a temperament association analysis in a Brahman population. Transversion effects on protein structure were evaluated in silico for each amino acid change model, revealing structural changes in the proteins of the HTR1B and SLC18A2 genes. The selected nsSNPs were genotyped in a Brahman population (n=138), and their genotypic effects on three temperament traits were analyzed: EV, PS, and TS. Only the SNP rs209984404-HTR1B (C/A) showed a significant association (P=0.0144) with PS. The heterozygous genotype showed a PS value 1.17 points lower than that of the homozygous CC genotype. The results showed that in silico analysis allows the selection of nsSNPs with the potential to change the protein, favoring the prioritization of SNPs associated with temperament in Brahman cattle.