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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #410482
Research Project: Genetic Improvement of Sorghum Traits that Advance Agricultural Productivity and Climate Resilience

Location: Plant Stress and Germplasm Development Research

Title: A large sequenced mutant library as a community resource for sorghum breeding and genomics

Author
item Xin, Zhanguo
item JIAO, YINPING - Texas Tech University
item Burow, Gloria
item Hayes, Chad
item Chen, Junping
item Pugh, Nicholas - Ace
item Ware, Doreen

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/13/2023
Publication Date: 4/2/2024
Citation: Xin, Z., Jiao, Y., Burow, G.B., Hayes, C.M., Chen, J., Pugh, N.A., Ware, D. 2024. A large sequenced mutant library as a community resource for sorghum breeding and genomics. Meeting Abstract.

Interpretive Summary:

Technical Abstract: We created a large mutant library of sorghum inbred line, BTx623 and sequenced 1256 lines. The library showed many valuable agronomic traits. We catalogued more than 10 million EMS-induced mutations, affecting almost all the genes in BTx623 genome excepting 517 short genes. We also found 610,320 mutations in the regulatory regions of 29,790 genes. We searched the mutation database and discovered a loss-of-function mutation in the gene for dhurrin synthesis, eliminating prussic acid, and new waxy sorghum mutants, among others. Furthermore, we developed a BSAseq pipeline to efficiently find the mutations responsible for important traits. The online databases SorghumBase (https://www.sorghumbase.org ) and SorbMuDB (https://www.depts.ttu.edu/igcast/SorbMutDB.php ) allow searching for mutations in any gene. Our mutant library is a great resource for sorghum breeding and genomics. We invite the sorghum community to use this free source for their research and breeding goals.