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ARS Home » Southeast Area » Stuttgart, Arkansas » Harry K. Dupree Stuttgart National Aquaculture Research Cntr » Research » Publications at this Location » Publication #410488
Research Project: Enhancing the Production of Hybrid Striped Bass Through Improved Genetics, Nutrition, Production Management, and Fish Health

Location: Harry K. Dupree Stuttgart National Aquaculture Research Cntr

Title: The development of triploidy induction methods for hybrid striped bass production in commercial aquaculture

Author
item GARCÍA-VÁZQUEZ, SAMUEL - University Of Arkansas At Pine Bluff
item BHATTARAI, SUJAN - University Of Arkansas At Pine Bluff
item Straus, David - Dave
item PERERA, DAVE - University Of Arkansas At Pine Bluff

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2023
Publication Date: 2/18/2024
Citation: García-Vázquez, S., Bhattarai, S., Straus, D.L., Perera, D. 2024. The development of triploidy induction methods for hybrid striped bass production in commercial aquaculture [abstract]. Aquaculture America 2024, February 18-21, 2024, San Antonio, Texas. p. 168.

Interpretive Summary:

Technical Abstract: Sunshine bass are a hybrid produced by crossing White bass females and Striped bass males. These bass hybrids are an important food fish in the United States. The average production cycle for this species can range from 10 to 20 months to reach a marketable size of 1.5 – 2.0 pounds. As with many commercially produced aquaculture species, controlling reproduction can be important to optimize growth and energy allocation efficiency in this production cycle. Triploidization is an effective method for production of sterile fishes. This project aims to evaluate three triploidy induction methods that have been used successfully with other commercial aquaculture species. Our goal is determining an optimum methodology for triploid production with special attention being placed on maximizing embryo survival and obtaining high triploidy induction in sunshine bass. The three methodologies include thermal shock, hydrostatic pressure shock, and electrical shock. In year one of this study hydrostatic pressure shocks were evaluated for the production of triploid sunshine bass embryos. Three pressures (6000, 7000, and 8000 PSI) and shock durations of 1.5, 2, 3, 4, and 5 minutes were evaluated. All shocks were administered 4 minutes post-fertilization. The produced fry were evaluated for triploidy using a flow cytometer. The initial results yielded very low survival and triploidy percentages. We believe the pressure may have been too intense and the duration too short. We will adjust the pressure shocks by lowering the pressure to 5000 PSI and lengthening the shock duration beyond five minutes. In year two, we will start the evaluation of thermal shocks and electrical shocks.