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ARS Home » Plains Area » El Reno, Oklahoma » Oklahoma and Central Plains Agricultural Research Center » Peanut and Small Grains Research Unit » Research » Publications at this Location » Publication #410494
Research Project: Genetic Improvement for Resistance to Aphids and Major Diseases in Wheat, Barley, and Sorghum in the Great Plains

Location: Peanut and Small Grains Research Unit

Title: Characterization of a new greenbug resistance gene Gb9 in a synthetic hexaploid wheat

Author
item Xu, Xiangyang
item Li, Genqiao
item Bai, Guihua
item BIAN, RUOLIN - Kansas State University
item Bernardo, Amy
item WATIRA, TEZERA - Oklahoma State University
item CARVER, BRETT - Oklahoma State University
item WU, YANQI - Oklahoma State University
item Elliott, Norman - Norm

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/10/2024
Publication Date: 5/23/2024
Citation: Xu, X., Li, G., Bai, G., Bian, R., Bernardo, A.E., Watira, T.W., Carver, B.F., Wu, Y., Elliott, N.C. 2024. Characterization of a new greenbug resistance gene Gb9 in a synthetic hexaploid wheat. Theoretical and Applied Genetics. 137. Article 140. https://doi.org/10.1007/s00122-024-04650-9.
DOI: https://doi.org/10.1007/s00122-024-04650-9

Interpretive Summary: Greenbug [Schizaphis graminum (Rondani)] is a serious insect pest that not only damages cereal crops, but also transmits several destructive viruses. The emergence of new greenbug biotypes in the field makes it urgent to identify novel greenbug resistance genes in wheat. CWI 76364, a synthetic hexaploid wheat (SHW) line, exhibits greenbug resistance. Evaluation of an F2:3 population from cross OK 14319 × CWI 76364 indicated that a dominant gene, designated Gb9, conditions greenbug resistance in CWI 76364. Genetic analysis delimited Gb9 to a 0.6-cM interval on the long arm of chromosome 7D and allelism tests indicated that Gb9 is a new greenbug resistance gene which confers resistance to greenbug biotypes C, E, H, I, and TX1. TX1 is one of the most widely virulent biotypes and has overcome most known wheat greenbug resistance genes. The introgression of Gb9 into locally adapted wheat cultivars is of economic importance, and the KASP markers developed in this study can be used to tag Gb9 in cultivar development.

Technical Abstract: (KASP) markers for genotyping the entire F2 population. Genetic analysis delimited Gb9 to a 0.6-cM interval flanked by KASP markers located at 599,835,668 bp (Stars-KASP872) and 600,471,081 bp (Stars-KASP881) on arm 7DL in which only nine high-confidence genes were identified. Gb9 was 0.5 cM distal to Stars-KASP872 and 0.5 cM proximal to Stars-KASP881. Allelism tests indicated that Gb9 is a new greenbug resistance gene which confers resistance to greenbug biotypes C, E, H, I, and TX1. TX1 is one of the most widely virulent biotypes and has overcome most known wheat greenbug resistance genes. The introgression of Gb9 into locally adapted wheat cultivars is of economic importance, and the KASP markers developed in this study can be used to tag Gb9 in cultivar development.