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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Plant Stress and Germplasm Development Research » Research » Publications at this Location » Publication #410582
Research Project: Development of Climate Resilient Germplasm and Management Tools for Sustainable Row Crop Production

Location: Plant Stress and Germplasm Development Research

Title: De novo genome sequences and assemblies from pathogenic fusarium oxysporum f. Sp. Vasinfectum isolates from California

Author
item Jobe, Timothy
item ELLIS, MARGARET - Fresno State University
item Ulloa, Mauricio

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/30/2023
Publication Date: 1/3/2024
Citation: Jobe, T.O., Ellis, M.L., Ulloa, M. 2024. De novo genome sequences and assemblies from pathogenic fusarium oxysporum f. Sp. Vasinfectum isolates from California. National Cotton Council Beltwide Cotton Conference.

Interpretive Summary:

Technical Abstract: Fusarium wilt of cotton (Gossypium spp.) is a vascular plant disease caused by the soilborne pathogen Fusarium oxysporum f. sp. vasinfectum (FOV). This widespread disease is responsible for substantial crop losses worldwide and is found in virtually all cotton producing regions. There are currently six recognized races of FOV. While multiple races of Fusarium oxysporum f. sp vasinfectum (FOV) have been identified, FOV race 4 (FOV4) is considered the most virulent cotton wilt pathogen in the US. FOV4 infestations have disrupted cotton production in California, far West Texas, and New Mexico. Recent efforts aimed at rapid identification and classification of FOV and FOV-specific races have focused on DNA sequencing of the translation elongation factor (EF-1a), phosphate permease (PHO), and ß-tubulin (BT) genes, and intergenic spacer (IGS) regions. Interestingly, these efforts have identified four distinct genotypes of FOV4 based on the absence (N biotype) or presence of the transposable element Tfo1 in the PHO gene (T biotype). The final two biotypes identified have additional independent MULE (MT biotype) or MITE (MiT biotype) insertions in the Tfo1 transposon. While the significance of these transposon insertions in the PHO gene is unclear, these genetic markers can be diagnostically useful to track and distinguish FOV4 populations. However, FOV races are continuing to change and there is currently an urgent need for additional genetic markers to improve and accelerate our diagnostic capabilities, especially for FOV4. To improve and accelerate our diagnostic capabilities for FOV4, whole genome sequencing of many diverse FOV and FOV4 isolates is needed. Here, we report the whole genome sequencing and assembly statistics of one FOV race 1 (FOV1) isolate and two FOV4 isolates from California.