Location: Dale Bumpers National Rice Research Center
Title: Discovery of a major gene behind prickles, an important plant innovation, in the large Solanum (Leptostemonum Clade)Author
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Huggins, Trevis |
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Eizenga, Georgia |
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SATTERLEE, JAMES - Cold Spring Harbor Laboratory |
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ALONSO, DAVID - University Of Valencia |
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GRAMAZIO, PIETRO - University Of Valencia |
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HE, JIA - Cold Spring Harbor Laboratory |
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ARRONES, ANDREA - University Of Valencia |
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VILLANUEVA, GLORIA - University Of Valencia |
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PLAZAS, MARIOLA - University Of Valencia |
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JENIKE, KATHARINE - Johns Hopkins University |
Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 2/1/2024 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Solanum is one of the largest genera in the plant kingdom with approximately 1,250 species. The largest monophyletic group within Solanum is the Leptostemonum Clade, with more than 500 species. A distinctive characteristic of this clade is the presence of epidermal prickles on vegetative organs; these are a synapomorphy of the Clade and represent a single origin evolutionary innovation in the genus. Prickles have been lost independently in several taxa. In cultivated species, breeders favored selection of prickleless (pl) materials during crop domestication. Interspecific mapping populations between pl eggplant (Solanum melongena) and wild prickled species revealed that the pl phenotype is recessive and consistent with one Mendelian locus. Advanced backcrosses of prickle-bearing wild species into the S. melongena background pointed to a region at the end of chromosome 6 containing the causal genetic locus. Fine mapping of the region narrowed the causal interval to a 600 kb genomic region containing 13 annotated genes that included a LONELY-GUY (LOG) cytokinin biosynthesis gene harboring a splice-site mutation. Other prickleless cultivated eggplants were found to have either splice-site mutations (S. macrocarpon) or a deletion (S. aethiopicum) in this gene, identifying it as PL. Sequencing of PL in prickleless species from the Leptostemonum clade revealed 15 independent mutations (i.e. frameshift, splice-site, TE insertion, and deletion alleles). The key role of PL in the development of prickles was further confirmed by CRISPR-Cas9 gene editing of PL in the prickly Australian wild species S. cleistogamum and S. prinophyllum, which recapitulated the phenotype. Our results indicate that PL may have had a major role in the evolution of an adaptive morphological novelty in the Leptostemonum Clade, as well as serving as a key genetic target during multiple instances of crop domestication. |