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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #411522
Research Project: Integration of Traditional Methods and Novel Molecular Strategies for Improving Disease Resistance and Input-use Efficiency in Peanut

Location: National Peanut Research Laboratory

Title: Draft genome sequence data of Nothopassalora personata, peanut foliar pathogen from Argentina

Author
item MONQUILLOT, JOAQUIN - Instituto Nacional Tecnologia Agropecuaria
item Arias De Ares, Renee
item Orner, Valerie
item Massa, Alicia
item Sobolev, Victor
item BERNARDI-LIMA, NELSON - Instituto Nacional Tecnologia Agropecuaria
item PAREDES, JUAN - Instituto Nacional Tecnologia Agropecuaria
item ODDINO, CLAUDIO - Consejo Nacional De Investigaciones Científicas Y Técnicas(CONICET)
item CARMONA, MARCELO - Universidad De Buenos Aires
item CONFORTO, CINTHIA - Faculty Of Agronomy And Veterinary Medicine

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/31/2024
Publication Date: 2/6/2024
Citation: Monquillot, J.H., Arias De Ares, R.S., Orner, V.A., Massa, A.N., Sobolev, V., Bernardi-Lima, N., Paredes, J., Oddino, C., Carmona, M., Conforto, C. 2024. Draft genome sequence data of Nothopassalora personata, peanut foliar pathogen from Argentina. Data in Brief. 53:Article 110158. https://doi.org/10.1016/j.dib.2024.110158.

Interpretive Summary: Peanut Late Leaf Spot is among the three top diseases that affect this crop in Argentina. To develop management practices the first step is to understand the pathogen. So far, only one isolate of Nothopassalora personata(n. personata) has been sequenced in the U.S.A, but none of them in Argentina. To begin studying the genetic diversity of N. personata we sequenced the genome of a type culture, and in this manuscript we highlight the genetic differences with the reference genome from U.S.A.

Technical Abstract: Late leaf spot (LLS) caused by the Ascomycete Nothopassalora personata (N.p.) (Syn. Cercosporidium personatum) is the main foliar disease of peanuts in Argentina and in peanut producing areas of the world, causing up to 70% yield losses. The extremely slow growth of this fungus in culture, that takes around one month to form a 1 cm colony (0.45 mm/day), and the lack of adequate young tissues from where to extract nucleic acids, have hindered genetic studies of this pathogen. Here, we report the first genome sequence of a N. personata isolate from South America, as well as genetic variants on its conserved genes, and the complete sequence of its mating-type locus MAT1-2 idiomorph. The N. personata isolate IPAVE 0302 was obtained from peanut leaves in Córdoba, Argentina.The whole genome sequencing of IPAVE 0302 was performed as paired end 150 bp NovaSeq 6000 and de novo assembled. Clean reads were mapped to the reference genome for this species NRRL 64463 and the genetic variants on highly conserved genes and throughout the genome were analyzed. Sequencing data were submitted to NCBI GenBank Bioproject PRJNA948451, accession number SRR23957761.