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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #411597
Research Project: Improving Dairy Cow Feed Efficiency and Environmental Sustainability Using Genomics and Novel Technologies to Identify Physiological Contributions and Adaptations

Location: Animal Genomics and Improvement Laboratory

Title: Effects of rumen metabolite butyric acid on bovine skeletal muscle satellite cells proliferation, apoptosis, and transcriptional states during myogenic differentiation

Author
item WANG, XIAOWEI - Ningxia University
item DING, YANGLING - Ningxia University
item LI, CHENHLONG - Ningxia University
item MA, QING - Ningxia University
item SHI, YUANGANG - Ningxia University
item Liu, Ge - George
item Li, Congjun - Cj
item KANG, XIAOLONG - Ningxia University

Submitted to: Domestic Animal Endocrinology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/10/2024
Publication Date: 10/11/2024
Citation: Wang, X., Ding, Y., Li, C., Ma, Q., Shi, Y., Liu, G., Li, C., Kang, X. 2024. Effects of rumen metabolite butyric acid on bovine skeletal muscle satellite cells proliferation, apoptosis, and transcriptional states during myogenic differentiation. Domestic Animal Endocrinology. https://doi.org/10.1016/j.domaniend.2024.106892.
DOI: https://doi.org/10.1016/j.domaniend.2024.106892

Interpretive Summary: Butyrate influences animal digestion and locomotor systems, but the impact of butyrate on the proliferation and differentiation of bovine skeletal muscle satellite cells remains unclear. This study aimed to elucidate the effects of butyric acid on the proliferation and differentiation of bovine skeletal muscle satellite cells. Transcriptome sequencing revealed differential expression of genes associated with skeletal muscle satellite cell differentiation and energy metabolism, enriching pathways such as Oxidative phosphorylation, MAPK, and Wnt signaling after being treated with butyrate. These findings offer valuable insights into the molecular mechanisms underlying butyric acid regulation of bovine skeletal muscle satellite cell proliferation and differentiation.

Technical Abstract: Butyric acid, a pivotal short-chain fatty acid in rumen digestion, profoundly influences animal digestive and locomotor systems. Extensive research indicates its direct or indirect involvement in the growth and development of muscle and fat cells. However, the impact of butyric acid on the proliferation and differentiation of bovine skeletal muscle satellite cells (SMSCs) remains unclear. This study aimed to elucidate the effects of butyric acid on SMSCs. After isolating, SMSCs were subjected to varying concentrations of sodium butyrate (NaB) during the proliferation and differentiation stages. Optimal treatment conditions (1 mM NaB for 2 days) were determined based on proliferative force, cell viability, and mRNA expression of proliferation and differentiation marker genes. Transcriptome sequencing was employed to screen for differential gene expression between 1 mM NaB-treated and untreated groups during SMSC differentiation. Results indicated that lower NaB concentrations (=1.0 mM) inhibited proliferation while promoting differentiation and apoptosis after a 2-day treatment. Conversely, higher NaB concentrations (=2.0 mM) suppressed proliferation and differentiation and induced apoptosis. Transcriptome sequencing revealed differential expression of genes associated with SMSC differentiation and energy metabolism, enriching pathways such as Oxidative phosphorylation, MAPK, and Wnt signaling. These findings offer valuable insights into the molecular mechanisms underlying butyric acid regulation of bovine SMSC proliferation and differentiation.