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Research Project: Intervention Strategies to Control and Eradicate Foreign Animal Diseases of Swine

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Title: Rapid detection and quick characterization of African swine fever virus using the Voltrax automated library preparation platform

Author
item O'DONNEL, VIVIAN - Animal And Plant Health Inspection Service (APHIS)
item PIERCE, JIM - Animal And Plant Health Inspection Service (APHIS)
item OSIPENKO, OLEG - Animal And Plant Health Inspection Service (APHIS)
item XU, LIZHE - Oak Ridge Institute For Science And Education (ORISE)
item BERNINGER, AMY - Animal And Plant Health Inspection Service (APHIS)
item LAKIN, STEVEN - Animal And Plant Health Inspection Service (APHIS)
item BARRETTE, ROGER - Kansas State University
item Gladue, Douglas
item FABURAY, BONTO - Animal And Plant Health Inspection Service (APHIS)

Submitted to: Viruses
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/1/2024
Publication Date: 5/5/2024
Citation: O'Donnel, V., Pierce, J., Osipenko, O., Xu, L., Berninger, A., Lakin, S., Barrette, R., Gladue, D.P., Faburay, B. 2024. Rapid detection and quick characterization of African swine fever virus using the Voltrax automated library preparation platform. Viruses. 16(5). Article 731. https://doi.org/10.3390/v16050731.
DOI: https://doi.org/10.3390/v16050731

Interpretive Summary: This report describes the use of an automated system to prepare ASFV DNA for sequencing for next generation sequencing, providing a full length sequence for African swine fever virus.

Technical Abstract: African swine fever virus (ASFV) is the causative agent of a severe and highly contagious viral disease affecting domestic and wild swine, with the current pandemic strain having a high mortality rate, severely impacting pig production, and for countries suffering outbreaks, the inability to export their pig products for international trade. Early detection and diagnosis of ASFV is necessary to control new outbreaks before disease spreads rapidly. One of the rate limiting steps to identify ASFV by next-generation sequencing platforms is library preparation. Here, we investigated the capability of the Oxford nanopore Voltrax platform for automated DNA library preparation, with down-stream sequencing on nanopore sequencing platforms to identify ASFV. Within minutes, DNA libraries prepared using Voltrax, generated near-full genome sequences of ASFV. Overall, our data highlights the use of the Voltrax, as a device for automated library preparation, coupled with sequencing on Nanopore devices, MinION, Mk1C for field sequencing or GridION within a laboratory setting. These results suggest Voltrax as a tool for library preparation that can be used for a rapid and real time detection of African swine fever in the event that ASFV is a possible cause for a disease case in swine.