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ARS Home » Pacific West Area » Logan, Utah » Poisonous Plant Research » Research » Publications at this Location » Publication #412315
Research Project: Developing Mitigation Strategies for Poisonous Plants in Livestock Production Systems

Location: Poisonous Plant Research

Title: Multiomics reveals mechanisms of Alternaria oxytropis inhibiting pathogenic fungi in Oxytropis ochrocephala

Author
item FU, KEYI - Lanzhou University
item SCHARDL, CHRISTOPHER - University Of Kentucky
item Cook, Daniel
item CAO, XUANLI - Lanzhou University
item LING, NING - Lanzhou University
item HE, CHUNYU - Lanzhou University
item WU, DANDAN - Lanzhou University
item XUE, LONGHAI - Lanzhou University
item LI, YANZHONG - Lanzhou University
item SHI, ZUNJI - Lanzhou University

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/4/2024
Publication Date: 1/17/2024
Citation: Fu, K., Schardl, C.L., Cook, D., Cao, X., Ling, N., He, C., Wu, D., Xue, L., Li, Y., Shi, Z. 2024. Multiomics reveals mechanisms of Alternaria oxytropis inhibiting pathogenic fungi in Oxytropis ochrocephala. Journal of Agricultural and Food Chemistry. 72(4):2397-2409. https://doi.org/10.1021/acs.jafc.3c09049.
DOI: https://doi.org/10.1021/acs.jafc.3c09049

Interpretive Summary: Endophytic fungi can benefit the host plant and increase the plant resistance. Now, there is no in-depth study of how Alternaria oxytropis (A. oxytropis) is enhancing the ability of inhibiting pathogenic fungi in Oxytropis ochrocephala (O. ochrocephala). In this study, the fungal community and metabolites associated with endophyte-infected (EI) and endophyte-free (EF) O. ochrocephala were compared by multiomics. The fungal community indicated that there was more A. oxytropis, less phylum Ascomycota, and less genera Leptosphaeria, Colletotrichum, and Comoclathris in the EI group. As metabolic biomarkers, the levels of swainsonine and apigenin-7-O-glucoside-4-O-rutinoside were significantly increased in the EI group. Through in vitro validation experiments, swainsonine and apigenin-7-O-glucoside-4-O-rutinoside can dramatically suppress the growth of pathogenic fungi Leptosphaeria sclerotioides and Colletotrichum americae-borealis by increasing the level of oxidative stress. This work suggested that O. ochrocephala containing A. oxytropis could increase the resistance to fungal diseases by markedly enhancing the content of metabolites inhibiting pathogenic fungi.

Technical Abstract: Endophytic fungi can benefit the host plant and increase the plant resistance. Now, there is no in-depth study of how Alternaria oxytropis (A. oxytropis) is enhancing the ability of inhibiting pathogenic fungi in Oxytropis ochrocephala (O. ochrocephala). In this study, the fungal community and metabolites associated with endophyte-infected (EI) and endophyte-free (EF) O. ochrocephala were compared by multiomics. The fungal community indicated that there was more A. oxytropis, less phylum Ascomycota, and less genera Leptosphaeria, Colletotrichum, and Comoclathris in the EI group. As metabolic biomarkers, the levels of swainsonine and apigenin-7-O-glucoside-4-O-rutinoside were significantly increased in the EI group. Through in vitro validation experiments, swainsonine and apigenin-7-O-glucoside-4-O-rutinoside can dramatically suppress the growth of pathogenic fungi Leptosphaeria sclerotioides and Colletotrichum americae-borealis by increasing the level of oxidative stress. This work suggested that O. ochrocephala containing A. oxytropis could increase the resistance to fungal diseases by markedly enhancing the content of metabolites inhibiting pathogenic fungi.