An overview of shoot tip cryopreservation

Authors: BETTONI, JEAN - Collaborator; CHEN, KATHERYN - Colorado State University; Volk, Gayle

Submitted to: Electronic Publication
Publication Type: Book / Chapter
Publication Acceptance Date: 3/19/2024
Publication Date: 3/19/2024
Citation: Bettoni, J.C., Chen, K., Volk, G.M. 2024. An overview of shoot tip cryopreservation. In: Volk, G.M., Chen, K., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/an-overview-of-shoot-tip-cryopreservation/

Interpretive Summary: The purpose of this eBook chapter is to present an overview of the steps involved in plant shoot tip cryopreservation and to highlight some of the fundamental concepts behind these procedures. It includes topics such as selecting plant materials, excising shoot tips, cryopreservation steps, and recovery and viability assessments. It describes options available for each step and how they can be optimized based on the type of plant materials that will be cryopreserved.

Technical Abstract: Educational/training resources are being developed for topics related to plant genetic resources management and use. This is an ebook chapter that will be published in an ebook titled "Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules". This ebook chapter is publicly available at: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/an-overview-of-shoot-tip-cryopreservation/