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Research Project: Intervention Strategies to Mitigate Avian Escherichia coli Infections and Antimicrobial Resistance in the Poultry Environment

Location: Poultry Research

Title: Complete genome sequences of two avian pathogenic Escherichia coli strains isolated from broilers exhibiting colibacillosis in Mississippi

Author
item JIA, LINAN - Mississippi State University
item ARICK II, MARK - Mississippi State University
item HSU, CHUAN-YU - Mississippi State University
item PETERSON, DANIEL - Mississippi State University
item Evans, Jeffrey - Jeff
item Robinson, Kelsy
item ADHIKARI, PRATIMA - Mississippi State University
item ZHANG, LI - Mississippi State University

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/18/2024
Publication Date: 4/30/2024
Citation: Jia, L., Arick Ii, M.A., Hsu, C., Peterson, D.G., Evans, J.D., Robinson, K., Adhikari, P., Zhang, L. 2024. Complete genome sequences of two avian pathogenic Escherichia coli strains isolated from broilers exhibiting colibacillosis in Mississippi. Microbiology Resource Announcements. 13:e01020-23. https://doi.org/10.1128/mra.01020-23.
DOI: https://doi.org/10.1128/mra.01020-23

Interpretive Summary: Development of novel, effective therapeutic agents against Avian Pathogenic Escherichia coli (APEC) is often hindered by the wide genetic diversity between individual isolates. Whole-genome sequencing of isolates allows veterinarians to make efficient disease control decisions. Making genome sequencing publicly available is vital as the scientific community works to better understand the disease mechanism of APEC. Here, we report the whole-genome sequences of Escherichia coli strains APEC-O2-MS1266 and APEC-O2-MS1657 isolated from the liver and heart of infected broilers in Mississippi State, US.

Technical Abstract: We report the whole-genome sequences of Escherichia coli strains APEC-O2-MS1266 and APEC-O2-MS1657 isolated from the liver and heart of infected broilers in Mississippi State, US. Genomic DNA was isolated from strains grown overnight in a 37' LB broth. Following quantity and quality assessment, DNA was fragmented to generated a mean fragment size of 12 - 15 kilobases for sequencing on a Nanopore GridION sequencer. A circular assembly was calculated for both strains. Sequences were annotated and multilocus sequence typing and serotyping were performed.