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ARS Home » Midwest Area » Lexington, Kentucky » Forage-animal Production Research » Research » Publications at this Location » Publication #413504
Research Project: Increasing Sustainability of Forage Production in Mid-South Agroecosystems

Location: Forage-animal Production Research

Title: Separation of mycotoxins in floral tissue of hemp infected by Fusarium graminearum [abstract]

Author
item Kagan, Isabelle
item SMITH, HENRY - University Of Kentucky
item GAUTHIER, NICOLE - University Of Kentucky

Submitted to: American Chemical Society SciMeetings
Publication Type: Abstract Only
Publication Acceptance Date: 5/21/2024
Publication Date: 8/18/2024
Citation: Kagan, I., Smith, H.S., Gauthier, N. 2024. Separation of mycotoxins in floral tissue of hemp infected by Fusarium graminearum [abstract]. American Chemical Society SciMeetings. https://scimeetings.acs.org/exhibit/Separation-mycotoxins-floral-tissue-hemp/4104837.

Interpretive Summary:

Technical Abstract: Fusarium graminearum, a grain pathogen, has been found to cause disease on hemp across the U.S. In grains, production of mycotoxins, particularly deoxynivalenol (DON), by F. graminearum is a major health concern for humans and livestock. Little is known about whether F. graminearum produces DON or related mycotoxins in hemp. A method is being developed to extract and quantify DON and several derivatives in the floral tissue of hemp infected with F. graminearum. Commercial standards of nivalenol (NIV), DON, DON-3-glucoside (D3G, a plant-produced derivative), and 3- and 15-acetyldeoxynivalenol (3-ADON and 15-ADON, respectively) were separated by high-performance liquid chromatography (HPLC) on a C18 column with an acetonitrile-water gradient and ultraviolet (UV) detection. External calibration curves with R2 > 0.99 were obtained for DON, NIV, D3G, 3-ADON, and 15-ADON. Air-dried hemp floral tissue was extracted with 84% acetonitrile. Solid-phase extraction removed polar interfering compounds and some of the cannabinoids. When hemp floral tissue infected with F. graminearum was extracted and separated, coinjections of 15-ADON and 3-ADON coeluted with sample peaks. The 3-ADON coeluted with a compound having a different absorbance maximum; hence, further resolution or cleanup is needed for 3-ADON identification. DON, NIV, and D3G were not detected in the tested samples at the detection limits set. The method will be used to quantify mycotoxins in field samples.