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ARS Home » Southeast Area » Stoneville, Mississippi » Southern Insect Management Research » Research » Publications at this Location » Publication #413584
Research Project: Insect Control and Resistance Management in Corn, Cotton, Sorghum, Soybean, and Sweet Potato, and Alternative Approaches to Tarnished Plant Bug Control in the Southern United States

Location: Southern Insect Management Research

Title: Insecticide Susceptibilities and Enzyme Activities of Four Stink Bug Populations in Mississippi, USA

Author
item Du, Yuzhe - Cathy
item Scheibener, Shane
item Zhu, Yu Cheng
item Allen, Clint
item Reddy, Gadi V.P.

Submitted to: Insects
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/10/2024
Publication Date: 4/12/2024
Citation: Du, Y., Scheibener, S.A., Zhu, Y., Allen, K.C., Reddy, G.V. 2024. Insecticide susceptibility and detoxifying enzyme activities of four stink bug populations on soybean in Mississippi, USA. Insects. 15/265. https://doi.org/10.3390/insects15040265.
DOI: https://doi.org/10.3390/insects15040265

Interpretive Summary: In Mississippi, the stink bug infesting soybean is mainly composed of the brown stink bug, southern green stink bug, green stink bug, and red banded stink bug. This study conducted spray bioassays to assess the resistance levels of these stink bugs to seven commonly used formulated insecticides. Stinks bugs were collected from soybeans in Leland during the fall of 2022 and 2023, as well as from wild host plants in Clarksdale, MS. While green stink bugs showed similar susceptibility across two years, red banded stink bug exhibited a slight increase in susceptible to neonicotinoids in 2023 compared to 2022. In 2022, the susceptibility raking among species was red banded stink bug = green stink bug = southern green stink bug, whereas in 2023, it was red banded stink bug = green stink bug << brown stink bug. Furthermore, enzyme activity analysis revealed no significant differences between southern green and green stink bug. However, compared to SIMRU-2023, populations of green stink bug and red banded stink bug from SIMRU-2022 showed elevated enzyme activities, highlighting potential variations in resistance mechanisms. These findings provide a foundation for monitoring and managing insecticide resistance in Mississippi soybean.

Technical Abstract: In Mississippi, the Pentatomidae complex infesting soybean is primarily comprised of Euschistus servus, Nezara viridula, Chinavia hilaris, and Piezodorus guildinii. This study employed spray bioassays to evaluate the resistance levels of these stink bugs to seven commonly used formulated insecticides: oxamyl, acephate, bifenthrin, '-cyhalothrin, imidacloprid, thiamethoxam, and sulfoxaflor. Stinks bugs were collected from soybean in Leland, MS, during the fall of 2022 and 2023, as well as from wild host plants in Clarksdale. There was no significant difference in the susceptibility of C. hilaris to insecticides between two years, whereas P. guildinii showed a slightly increased susceptibility to neonicotinoids in 2023. Among all four stink bug species, susceptibility in 2022 was ranked as P. guildinii = C. hilaris = N. viridula, while in 2023, it was ranked as P. guildinii = C. hilaris << E. Servus, Additionally, populations of E. Servus and P. guildinii collected from Clarksdale, MS exhibited high tolerance to pyrethroids and neonicotinoids. Moreover, populations of E. Servus and P. guildinii from SIMRU-2022 and Clarksdale-2023 showed elevated esterase and cytochrome P450 activity, respectively. These findings from spray bioassays and enzyme activity analyses offer a baseline for monitoring insecticide resistance in Pentatomidae and can guide insecticide resistance management strategies for Mississippi soybean.