Longitudinal assessment of Salmonella enterica prevalence and concentration throughout the gastrointestinal tract of finishing beef steers with and without liver abscesses

Authors: DORNBACH, COLTEN - Texas Tech University; Broadway, Paul; LINE, DALTON - Texas Tech University; HANRATTY, ASHLEE - Texas Tech University; CHILDRESS, KALLIE - Texas Tech University; THOMPSON-SMITH, AUBREY - Texas Tech University; MANAHAN, JEFF - Texas Tech University; MCDANIEL, ZACH - Texas Tech University; CANTERBURY, LANDON - Texas Tech University; Sanchez, Nicole; GALYEAN, MICHAEL - Texas Tech University; HALES, KRISTIN - Texas Tech University

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Our objective was to longitudinally assess the presence and quantity of Salmonella enterica throughout the gastrointestinal tract of finishing beef steers with and without liver abscesses (LA). Crossbred steers (n = 225; initial body weight = 353 ± 39 kg) were used in an observational case-control design with individual steer designated as the experimental unit. Nasal, ruminal fluid, and fecal samples were collected at feedlot arrival, one week after adaptation to the finishing diet, and the day before harvest at a commercial abattoir. Healthy and abscessed liver tissue samples were collected at harvest. Steers were fed a steam-flaked corn-based diet without tylosin phosphate. After harvest, LA prevalence was determined, and steers were sorted into those with and without LA. Data were analyzed using the GLIMMIX procedure of SAS. Overall LA prevalence was 18.7% (n = 42). Nasal Salmonella prevalence was 34.4% but did not differ between steers with or without LA (P = 0.73), and nasal Salmonella concentrations did not differ between treatments (P = 0.85) or collection day (P = 0.50). Ruminal fluid Salmonella prevalence was 73.7% but did not differ between treatments (P = 0.83). From feedlot arrival to harvest, ruminal fluid Salmonella concentration and prevalence decreased (P < 0.01). Fecal Salmonella prevalence tended (P = 0.09) to be 6.4% greater in steers with LA. Conversely, fecal Salmonella concentration tended (P = 0.07) to be 5.9% greater in steers without LA. Fecal Salmonella prevalence was greatest after transition to the finishing diet (P < 0.01), whereas fecal Salmonella concentration was greatest before harvest (P < 0.01). Liver Salmonella prevalence was 9.8% and 6.5% for steers with and without LA, respectively, but did not differ between treatments (P = 0.47). Similarly, liver Salmonella concentrations did not differ in steers with or without LA (P = 0.18). Overall, these data suggest Salmonella concentration and prevalence were affected by time on feed, but not indicative of LA formation.