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ARS Home » Southeast Area » Auburn, Alabama » Soil Dynamics Research » Research » Publications at this Location » Publication #414391
Research Project: Integrated Crop Disease Mitigation through Improved Understanding of Relationships between Genetics, Environment, and Management

Location: Soil Dynamics Research

Title: Yeast two-hybrid screening for cotton leafroll dwarf virus-Aphis gossypii interactions

Author
item MUELLER, NICHOLAS - Auburn University
item Zhao, Chaoyang

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2024
Publication Date: 5/17/2024
Citation: Mueller, N., Zhao, C. 2024. Yeast two-hybrid screening for cotton leafroll dwarf virus-Aphis gossypii interactions. Plants, Insects and Microbes Symposium, Auburn, AL. May 17, 2024.

Interpretive Summary:

Technical Abstract: Cotton leafroll dwarf virus (CLRDV) is a globally occurring Polerovirus and poses a potential risk to cotton production in the United States. Aphis gossypii, the cotton-melon aphid, is the only known insect vector that transmits CLRDV, however, the molecular mechanisms of virus transmission remain unrevealed. The genome of CLRDV is composed of open reading frames (ORFs) for seven proteins. Based on previous studies on other Polerovirus species, ORF3 encoding for P3 and ORF5 encoding for the read-through domain (RTD) of P3-5 are likely to be responsible for the transmission by A. gossypii. A yeast two-hybrid (Y2H) assay is used to identify protein interactors between CLRDV and A. gossypii. Four bait constructs were made to express the full ORF3 (P3), the C-terminal fragment of ORF3 (P3C), the full ORF5 (RTD), and the N-terminal fragment of RTD (NRTD), and were used to screen an A. gossypii cDNA library. Nine candidate proteins in A. gossypii have been identified that appeared to interact with at least one of the CLRDV bait constructs. Through pairwise Y2H test, the number of candidates has been narrowed, and their interactions with CLRDV await further validation and functional assays. The identification of these CLRDV-A. gossypii protein-protein interactions could lead to the development of new methods to block CLRDV transmission by A. gossypii.