Ultra-long sequencing experiences with a variety of mammalian sample types

Authors: Kuhn, Kristen; Smith, Timothy - Tim

Submitted to: World Wide Web
Publication Type: Other
Publication Acceptance Date: 4/3/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Ultra-long sequencing technology is a crucial component of genome assembly. Oxford Nanopore's technology is utilized in the generation of ultra-long sequencing reads. Currently, generating ultra-long sequence data is challenging. Results vary in success reliant upon different sample types and sequencing approaches by different research groups. Output generated via available sequencing technologies varies by length and cost, with the cheapest being Illumina short read (a few hundred bases of data) sequencing that isn’t very useful for genome assembly, Pacific Biosciences HiFi long read sequencing (10,000-50,000 bases) that is more expensive but can be used to produce fairly contiguous genome assemblies, and Oxford Nanopore“ultra-long” sequencing (greater than 100,000 bases) that is necessary if the goal is to get complete genome assemblies that accurately represent entire chromosomes without gaps or errors. The purpose of this brief talk is to discuss user experiences with the Oxford Nanopore technology and to identify ways to improve yield and read lengths of the ultra-long sequence data to improve animal genome assemblies to get complete chromosomes.