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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #414793
Research Project: Improvement of Disease and Pest Resistance in Barley, Durum, Oat, and Wheat Using Genetics and Genomics

Location: Cereal Crops Improvement Research

Title: Identification of two QTL for Hessian fly resistance in tetraploid wheat

Author
item Peters Haugrud, Amanda
item Anderson, Kirk
item HARRIS, MARION - North Dakota State University
item Faris, Justin
item Xu, Steven

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/17/2024
Publication Date: 9/22/2024
Citation: Peters Haugrud, A.R., Anderson, K.M., Harris, M., Faris, J.D., Xu, S.S. 2024. Identification of two QTL for Hessian fly resistance in tetraploid wheat. Meeting Abstract. Poster No. 260.

Interpretive Summary:

Technical Abstract: North Dakota is the largest durum (Triticum durum Desf.) producing state within the United States. Hessian fly (HF, Mayetiola destructor) is a major pest on wheat, and is often observed in wheat growing regions worldwide. We evaluated a recombinant inbred line population (referred to as BP025) consisting of 200 lines derived from a cross between the North Dakota durum wheat variety Ben and the cultivated emmer wheat accession PI 41025 for resistance to the Great Plains biotype of HF. The BP025 population was evaluated under greenhouse/growth chamber conditions and 14 plants per line were scored 10 to 14 days post egg laying. Seedlings were evaluated for stunting score, larvae mortality, and percent of resistant plants. No significant quantitative trait loci (QTLs) were associated with stunting score. Larvae mortality and percent of resistant plants were highly correlated (0.93), with the same two QTL regions associated with each trait. QHf.fcu-2A, located on the distal end of chromosome arm 2AL, explained 28.67 and 33.47 percent of the phenotypic variation in larvae mortality and precent resistant plants, respectively. PI 41025 alleles at QHf.fcu-2A resulted in increased HF resistance. The other QTL, QHf.fcu-3B, mapped to the distant end of chromosome arm 3BS and explained 21.76 and 19.41 percent of the phenotypic variation in larvae mortality and precent resistant plants, respectively. Ben was the donor parent at QHf.fcu-3B for increased resistance. The QTL on 3BS is within the same region as H35, originally mapped in winter wheat, and the H35 KASP markers KASP-3B3797431 and KASP-3B4525164 were added to the BP025 map and mapped to the distal end of chromosome arm 3BS and are within the confidence region of QHf.fcu-3B. Further work is needed to determine if QHf.fcu-3B is H35 or a novel HF resistance gene. The QTL on chromosome arm 2AL mapped in the same region as QH.icd-2A, which was also identified in cultivated emmer. The flanking SNP markers for QH.icd-2A and the proximal marker for QHf.fcu-3B have been converted to KASP to be used for screening and we have genotyped the Global Durum Panel (GDP) with these three markers. Currently, screening of the GDP for HF resistance to the Great Plains biotype is ongoing. The work here presents two HF resistant QTL, QHf.fcu-2A and QHf.fcu-3B, which can be used in durum breeding programs and the tools to screen for these QTL and introgress them via marker-assisted selection.