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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #415386
Research Project: Improvement of Disease and Pest Resistance in Barley, Durum, Oat, and Wheat Using Genetics and Genomics

Location: Cereal Crops Improvement Research

Title: Genetic mapping of a recessive resistance to stripe rust in barley

Author
item KAUR, SARABJIT - North Dakota State University
item LIU, ZHAOHUI - North Dakota State University
item Yang, Shengming

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2024
Publication Date: 6/10/2024
Citation: Kaur, S., Liu, Z., Yang, S. 2024. Genetic mapping of a recessive resistance to stripe rust in barley. Meeting Abstract. 2024 Annual Meeting of the North Central Division of the American Phytopathological Society.

Interpretive Summary:

Technical Abstract: Barley (Hordeum vulgare L.) is one of the most important cereal crops grown worldwide. Stripe rust caused by Puccinia striiformis f. sp. hordei (Psh) is one of the major barley diseases in the northwestern United States. This disease can cause seed quality reductions and yield losses reaching up to 70% in regions with cool and wet climates and is managed via targeted breeding efforts which aim to stack resistance quantitative trait loci (QTL). One recessive gene, named rps.gz which was reported in the Ethiopia cultivar Grannelose Zweizeilige (GZ), confers broad-spectrum resistance to stripe rust. Here, we developed a Steptoe x Grannelose Zweizeilige (GZ) biparental population (F2) and performed QTL analysis. A selected panel of 196 F2 progenies were phenotyped with Psh race 33 at the seedling stage in the greenhouse and were genotyped with the 20K barley SNP markers. The obtained segregation ratio for resistance/susceptible fits 1:3, indicating that the disease phenotype is controlled by a single dominant susceptibility gene. Interval mapping revealed a single QTL located on chromosome 4H, with a logarithm of the odds (LOD) score of 8.9. This QTL was flanked by two markers, AVRIG07556 and AVRIG07785, which delimited the gene to a 32-Mb region. Molecular markers were designed to narrow down the gene region. We aim to pinpoint this QTL, design gene-specific/diagnostic markers, and clone the gene, thus providing a valuable tool for breeding programs to control stripe rust in barley production.