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ARS Home » Pacific West Area » Albany, California » Plant Gene Expression Center » Research » Publications at this Location » Publication #415839
Research Project: Enhancing Crop Resilience to Biotic and Abiotic Stress Through Understanding the Microbiome and Immune Signaling Mechanisms

Location: Plant Gene Expression Center

Title: Clathrin light chains negatively regulate plant immunity by highjacking the autophagy pathway

Author
item LAN, HU-JIAO - Zhejiang Normal University
item RAN, JIE - Zhejiang Normal University
item WANG, WEN-XU - Zhejiang Normal University
item ZHANG, LEI - Zhejiang Normal University
item WU, NI-NI - Zhejiang Normal University
item ZHAO, YA-TING - Zhejiang Normal University
item HUANG, MIN-JUN - Zhejiang Normal University
item NI, MIN - Zhejiang Normal University
item LIU, FEN - Chinese Academy Of Agricultural Sciences
item CHENG, NINGHUI - Baylor College Of Medicine
item NAKATA, PAUL - Baylor College Of Medicine
item PAN, JIANWEI - Lanzhou University
item WHITHAM, STEVEN - Iowa State University
item Baker, Barbara
item LIU, JIAN-ZHONG - Zhejiang Normal University

Submitted to: Plant Communications
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/28/2024
Publication Date: 7/8/2024
Citation: Lan, H., Ran, J., Wang, W., Zhang, L., Wu, N., Zhao, Y., Huang, M., Ni, M., Liu, F., Cheng, N., Nakata, P.A., Pan, J., Whitham, S.A., Baker, B.J., Liu, J. 2024. Clathrin light chains negatively regulate plant immunity by highjacking the autophagy pathway. Plant Communications. 5. Article 100937. https://doi.org/10.1016/j.xplc.2024.100937.
DOI: https://doi.org/10.1016/j.xplc.2024.100937

Interpretive Summary: Clathrin plays a critical role in clathrin-mediated endocytosis (CME) in plants, and it is also required for autophagy in mammals. However, the functional interconnection of clathrin with autophagy, and its role in plant defense have not been established in plants. Here, we demonstrated that loss of function of CLATHRIN LIGHT CHAIN (CLC) subunit 2 and 3 resulted in Salicylic acid (SA)- and Reactive oxygen species (ROS)-dependent autoimmune responses in Arabidopsis, which are hallmarks of the AUTOPHAGY-RELATED GENE (ATG) mutants and thus linking clathrin-mediated endocytosis (CME) to autophagy and plant defense.

Technical Abstract: Clathrin plays a critical role in clathrin-mediated endocytosis (CME) in plants, and it is also required for autophagy in mammals. However, the functional interconnection of clathrin with autophagy, and its role in plant defense have not been established in plants. Here, we demonstrated that loss of function of CLATHRIN LIGHT CHAIN (CLC) subunit 2 and 3 resulted in Salicylic acid (SA)- and Reactive oxygen species (ROS)-dependent autoimmune responses in Arabidopsis, which are hallmarks of the AUTOPHAGY-RELATED GENE (ATG) mutants and thus linking clathrin-mediated endocytosis (CME) to autophagy and plant defense.