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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Exotic & Emerging Avian Viral Diseases Research » Research » Publications at this Location » Publication #416886
Research Project: Control Strategies to Prevent and Respond to Diseases Outbreaks Caused by Avian Influenza Viruses

Location: Exotic & Emerging Avian Viral Diseases Research

Title: Sequencing of historic samples provide complete coding sequences of chicken calicivirus from the United States

Author
item GORAICHUK, IRYNA - Orise Fellow
item DAVIS, JAMES - Georgia Poultry Laboratory Network
item AFONSO, CLAUDIO - Base2bio
item Suarez, David

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/5/2024
Publication Date: 9/12/2024
Citation: Goraichuk, I., Davis, J.F., Afonso, C., Suarez, D.L. 2024. Sequencing of historic samples provide complete coding sequences of chicken calicivirus from the United States. Microbiology Resource Announcements. 0:e00777-24. https://doi.org/10.1128/mra.00777-24.
DOI: https://doi.org/10.1128/mra.00777-24

Interpretive Summary: Next Generation Sequencing (NGS) is a powerful new tool that allows you to sequence viruses in clinical samples using a random amplification approach. Using random amplification can potentially sequence any virus in the sample if the virus is in high enough concentration without any prior knowledge that the virus was in the sample. Using this NGS approach we were able to sequence the full coding sequence of two caliciviruses from clinical samples from chickens. Having this sequence information will improve our ability to find and detect similar viruses from poultry samples and help determine if these viruses are important disease pathogens.

Technical Abstract: Here, we report the first complete coding genomic sequences of two chicken caliciviruses from U.S. poultry flocks in 2003 and 2004. They show the same genomic organization as that of other members of the Bavovirus genus and have the highest nucleotide identity (~88%) with strains from clinically normal chickens from Germany in 2004 and Netherlands in 2019.