Skip to main content
ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » Veterinary Pest Genetics Research Unit » Research » Publications at this Location » Publication #417285
Research Project: Genetics of Veterinary Pests

Location: Veterinary Pest Genetics Research Unit

Title: Horn fly transcriptome data of 10 populations from the southern United States Supplementary Data

Author
item Bendele, Kylie

Submitted to: Mendeley Data
Publication Type: Database / Dataset
Publication Acceptance Date: 2/5/2023
Publication Date: 5/13/2023
Citation: Bendele, K.G. 2023. Horn fly transcriptome data of 10 populations from the southern United States Supplementary Data. Mendeley Data. https://doi.org/10.1016/j.dib.2023.109272.
DOI: https://doi.org/10.1016/j.dib.2023.109272

Interpretive Summary: Pesticide usage for the control of horn flies in livestock has led to resistance in multiple classes of these chemicals. We sequenced ten populations of horn flies with varying degrees of resistance to study the different pesticide resistance seen among them using Illumina paired-end HiSeq. De novo assembly of each population’s transcriptome was conducted using the CLC Genomics Workbench 8.0.1 De Novo Assembler and annotation was done using Blast2GO PRO version 5.2.5. This research was reported in full in Bendele et al. Data in Brief and the supplemental data produced from this manuscript can be found here.

Technical Abstract: Supplemental data for Bendele et al. Data in Brief: Haematobia irritans irritans (Linnaeus, 1758: Diptera: Muscidae), the horn fly, is an external parasite of penned and pastured livestock that causes a major economic impact on cattle production worldwide. Pesticides such as synthetic pyrethroids and organophosphates are routinely used to control horn flies; however, resistance to these chemicals has become a concern in several countries. To further elucidate the molecular mechanisms of resistance in horn fly populations, we sequenced the transcriptomes of ten populations of horn flies from the southern US possessing varying degrees of pesticide resistance using an Illumina paired-end HiSeq approach, followed by de novo assembly of the transcriptomes using CLC Genomics Workbench 8.0.1 De Novo Assembler using multiple kmers, and annotation using Blast2GO PRO version 5.2.5. The raw sequence reads are archived in the Sequence Read Archive (SRA) and assembled population transcriptomes in the Transcriptome Shotgun Assembly (TSA) at the National Center for Biotechnology Information (NCBI).