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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #417653
Research Project: Broadening and Strengthening the Genetic Base of Rice for Adaptation to a Changing Climate, Crop Production Systems, and Markets

Location: Dale Bumpers National Rice Research Center

Title: Mapping yield related traits in a suite of six oryza rufipogon species complex chromosome segment substitution line libraries in Indica and Japonica rice backgrounds

Author
item Eizenga, Georgia

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 8/5/2024
Publication Date: 9/6/2024
Citation: Eizenga, G.C. 2024. Mapping yield related traits in a suite of six oryza rufipogon species complex chromosome segment substitution line libraries in Indica and Japonica rice backgrounds. Abstract. 21st International Symposium on Rice Functional Genomics, September 9-11, 2024, Little Rock, Arkansas.

Interpretive Summary:

Technical Abstract: The two varietal groups of cultivated rice (Oryza sativa) are indica and japonica. The rice ancestral species are O. rufipogon and O. nivara, collectively identified as the Oryza rufipogon species complex (ORSC) and are an underutilized resource for rice improvement. To make the ORSC genepool more accessible, three genotypically and phenotypically diverse ORSC accessions were selected as donors, OrA (W1944) from China, OrB (IRGC106148) from Loas and OrC (IRGC105567) from Indonesia, to develop chromosome segment substitution line (CSSL) libraries from crosses with IR64, an IRRI indica long grain and Cybonnet, a U.S. tropical japonica long grain. This study was conducted to characterize the libraries for yield-related traits and demonstrate how substitution mapping is used to discover the underlying genes. IR64 and 219 single IR64 CSSL plants from the three IR64 libraries were grown in the greenhouse and characterized for six agronomic, two panicle architecture and five seed traits. Cybonnet and 212 Cybonnet CSSLs from the three libraries were evaluated in replicated field studies for six agronomic, six panicle architecture and eight seed traits. Across the Cybonnet libraries, 62 CSSLs were significantly different from Cybonnet for one or more traits. To ascertain the chromosome region and underlying candidate gene(s) causing these differences, substitution mapping was performed with previously reported genotypes and results compared to substitution mapping with the IR64 libraries. Mapping will be illustrated using the CSSLs which had delayed heading under long days and revealed five genes, OsMADS50, RFT1, HD3A, SE1 and GHD7, associated with rice flowering time pathways.