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ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #418084
Research Project: Broadening and Strengthening the Genetic Base of Rice for Adaptation to a Changing Climate, Crop Production Systems, and Markets

Location: Dale Bumpers National Rice Research Center

Title: Functional analysis of a new allele of the broad-spectrum rice blast resistance gene Ptr in weedy species of rice

Author
item OSAKINA, ARON - Washington University
item OLSEN, KENNETH - Washington University
item Jia, Yulin

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 8/14/2024
Publication Date: 8/14/2024
Citation: Osakina, A., Olsen, K., Jia, Y. 2024. Functional analysis of a new allele of the broad-spectrum rice blast resistance gene Ptr in weedy species of rice. Abstract. 2024 International Symposium on Rice Functional Genomics, September 9-11, 2024. Little Rock/Stuttgart, Arkansas.

Interpretive Summary:

Technical Abstract: Rice blast caused by the fungus Magnaporthe oryzae is the most devasting disease in rice growing regions. The use of blast resistance (R) genes is the most efficient method to manage blast disease. The major blast R gene Ptr has recently been deployed in US rice varieties to control blast disease. Weedy red rice is a pest that aggressively competes with cultivated rice and has evolved unique ability to fight pathogens that can be exploited for crop protection. The Ptr gene in cultivated rice confers broad spectrum resistance to most common US blast races except the races, IE1k and IB33. IB33 is a laboratory strain that has not been found in any commercial rice fields. However, the Ptr gene in Black Hull Awn (BHA) weedy confers resistance to IB33. The mechanism in which the Ptr gene confer blast resistance remains unknown. In this study, we used polymerase chain reaction (PCR) method to amplify the coding region of the Ptr gene using the cDNA derived from a genotype of BHA. Alignment results using the Ptr proteins from Katy and Nipponbare rice varieties, revealed a predicted loss of 39 amino acids near the N-terminal region in the Ptr protein in US Black Hull weedy rice. We hypothesize that, this deletion could be crucial in triggering effective disease resistance. The molecular mechanism of the Ptr gene mediated blast resistance fungal and other plant components involved in signaling pathways are being sought using the yeast two hybrid system and progress will be presented.