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ARS Home » Southeast Area » Little Rock, Arkansas » Arkansas Children's Nutrition Center » Microbiome and Metabolism Research » Research » Publications at this Location » Publication #420157
Research Project: Impact of Maternal Influence and Early Dietary Factors on Child Growth, Development, and Metabolic Health

Location: Microbiome and Metabolism Research

Title: Maternal High-Fat Diet-Induced Obesity in Offspring: Unraveling Adipose Tissue Dysfunction Mediated by Increased Heat Shock Proteins

Author
item WANKHADE, UMESH - Arkansas Children'S Nutrition Research Center (ACNC)
item PAZ, HENRY - University Arkansas For Medical Sciences (UAMS)
item BUDDHA, LASYA - Arkansas Children'S Nutrition Research Center (ACNC)
item LAM, TIANFU - Arkansas Children'S Nutrition Research Center (ACNC)
item LOY, HANNAH - University Arkansas For Medical Sciences (UAMS)
item PILKINGTON, ANNA-CLAIRE - University Arkansas For Medical Sciences (UAMS)
item ZHONG, YING - University Arkansas For Medical Sciences (UAMS)
item SIKES, JAMES - Arkansas Children'S Nutrition Research Center (ACNC)
item SHANKAR, KARTIK - University Of Colorado
item ANDRES, ALINE - Arkansas Children'S Nutrition Research Center (ACNC)

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/11/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Objectives: Maternal weight and diet prior to and during pregnancy significantly affect offspring metabolic health. This study aimed to assess the sex-specific impact of maternal HFD-induced obesity on offspring adiposity, glucose metabolism, and lipid profiles. Methods: Female C57BL6/J mice were fed low or high-fat diets (10% or 45% calories) for 12 weeks. After mating, dams had access to low-fat or HFD during pregnancy and lactation. Post-weaning, offspring had control diet and HFD for 6 weeks. Assessments included body composition, weight, glucose homeostasis, and indirect calorimetry. Adipose tissue (AT) depots were analyzed via histology and RT-PCR. In a separate experiment, brown (wild-type mouse) and white (3T3L1) cell lines underwent adipogenic differentiation and bioenergetics assays. Umbilical cord-derived mesenchymal stem cells (UCMSC) from overweight/obese mothers (n=60) were used to assess gene expression associations with maternal BMI. Results: Male offspring born to HFD fed dams, displayed greater weight gain than females (maternal diet .sex (MD .S), p < 0.05)). Male offspring of HFD-fed dams exhibited elevated fat mass (MD, p<0.05) and glucose intolerance (MD .S, p <0.05), while maternal HFD induced differential cholesterol and lipoprotein profiles in males (MD .S, p < 0.05). Adipogenic gene expression and hypertrophy in inguinal white AT were more prominent in males, while heat shock protein (HSP) expression varied with maternal diet and sex (MD .S, p < 0.05). In vitro, brown adipocytes (BA) displayed lower expression of HSPs as differentiation progressed vs higher expression of HSPs in white adipocytes (WA) (p < 0.05). Induction of HSPs increased oxygen consumption rate in WA but not in brown adipocytes (p < 0.05). Additionally, HSP90ab1 gene expression tended to positively correlate with UCMSC of male infant origin (r = 0.34, p < 0.07) whereas no correlations were observed in female infant. Conclusion: In summary, maternal HFD induces sex-specific effects in offspring. Male offspring born to HFD-fed dams show differential HSP expression in adipose tissue. Differentiated UCMSCs suggest an association between HSP90ab1 expression and maternal body mass index, indicating a potential link between maternal diet, HSPs, and adipose tissue.