Influence of maternal supplementation with vitamins, minerals, and (or) protein/energy on placental development and angiogenic factors in beef heifers during pregnancy

Authors: DAVILA-RUIZ, B - North Dakota State University; DAHLEN, C - North Dakota State University; MCCARTHY, K - University Of Nebraska; CATON, J - North Dakota State University; HURLBERT, J - North Dakota State University; BAUMGAERTNER, F - North Dakota State University; MENEZES, A - South Dakota State University; DINIZ, W - Auburn University; UNDERAHL, S - North Dakota State University; KIRSCH, J - North Dakota State University; SEDIVEC, K - North Dakota State University; BOCHANTIN, K - North Dakota State University; BOROWICZ, P - North Dakota State University; CANOVAS, S - Universidad De Murcia; REYNOLDS, L - North Dakota State University

Submitted to: Veterinary Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/29/2024
Publication Date: 3/2/2024
Citation: Davila-Ruiz, B.J., Dahlen, C.R., Mccarthy, K.L., Caton, J.S., Hurlbert, J.L., Baumgaertner, F., Menezes, A.C., Diniz, W.J., Underahl, S.R., Kirsch, J.D., Sedivec, K.K., Bochantin, K.A., Borowicz, P.P., Canovas, S., Reynolds, L.P. 2024. Influence of maternal supplementation with vitamins, minerals, and (or) protein/energy on placental development and angiogenic factors in beef heifers during pregnancy. Veterinary Sciences. 11:111. https://doi.org/10.3390/vetsci11030111.
DOI: https://doi.org/10.3390/vetsci11030111

Interpretive Summary: Understanding placental vascularity is vital for ensuring the proper nourishment of the fetus and, therefore, a healthy offspring. We aimed to investigate the impact of vitamin and mineral supplementation and/or different rates of body weight gain on placental vascularity in beef heifers. To this end, in the first experiment, heifers were divided into groups that received vitamin and mineral supplementation or did not at least 72 days before breeding. At breeding, they were further divided into low or moderate-weight gain groups, resulting in four different treatments maintained until day 83 of pregnancy when tissue collection was performed. In the second experiment, another group of heifers received a basal diet or a diet with vitamin and mineral supplementation from breeding until parturition. We evaluated placental blood vessel density in both experiments and the placental expression of genes related to blood vessel growth in the first experiment. Results showed that supplementation and the rate of body weight gain during early pregnancy did not significantly affect placental vascularity or the expression of angiogenic factors. On the other hand, placental vascularity measured at parturition was increased in the fetal placenta of the supplemented group. These findings suggest that supplementation with vitamins and minerals throughout all gestation may impact placental function at a later stage of pregnancy.

Technical Abstract: The effect of vitamins and minerals supplementation (VTM) and/or two rates of body weight gain (GAIN) on bovine placental vascular development and angiogenic factors gene expression were evaluated in two experiments: In Exp. 1, crossbred Angus heifers (n = 34) were assigned to VTM/NoVTM treatments at least 71 days before breeding to allow changes in the mineral status. At breeding, through artificial insemination (AI), heifers were assigned to low-gain (LG) 0.28 kg/d or moderate-gain (MG) 0.79 kg/d treatments, resulting in NoVTM-LG (Control; n = 8), NoVTM-MG (n = 8), VTM-LG (n = 9), and VTM-MG (n = 9) until day 83 of gestation; In Exp. 2, crossbred angus heifers (n = 28), were assigned to control (CON; n = 12), receiving a basal total mixed ration (TMR) or TMR + VTM (VTM; n = 16) from breeding until parturition. Placentomes from Exp. 1 and cotyledons (COT) from Exp. 2 were evaluated by immunohistochemistry for COT vascular density area. COTs from Exp. 1 were evaluated for angiogenic factor (ANGPT-1, ANGPT-2, eNOS2, eNOS3, FLT1, KDR, TEK, VEGFA) gene expression. In Exp. 1, COT vascularity was not affected by the interaction of VTM and GAIN (p = 0.67) or the main effects of VTM (p = 0.50) and GAIN (p = 0.55). Likewise, angiogenic factors were not differentially expressed between treatments (p < 0.05). In Exp. 2, COT vascularity was greater in VTM vs. CON (p = 0.07). In conclusion, there is a suggested later-stage influence of vitamin and mineral supplementation on placental vascularity, emphasizing the importance of supplementation beyond early pregnancy.