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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Publications at this Location » Publication #422318
Research Project: Environmental and Management Influences on Animal Productivity and Well-Being Phenotypes

Location: Livestock Issues Research

Title: The evolving metabolome of the porcine longissimus dorsi following a prenatal LPS challenge or vaccine.

Author
item DOBBINS, THOMAS - Texas Tech University
item HERNANDEZ, M - Texas Tech University
item PUTNAM, MARGARET - University Of Missouri
item SWANSON, REBECCA - North Dakota State University
item MURPHY, ISABELLA - University Of Missouri
item EDWARDS, JESSIE - University Of Missouri
item Broadway, Paul
item Sanchez, Nicole
item PETRY, AMY - University Of Missouri
item LEGAKO, JERRAD - Texas Tech University

Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 2/11/2025
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Previously, the administration of LPS to sows during gestation was shown to alter the metabolome and muscle ultrastructure of offspring at weaning. The aim of this study was to evaluate the evolving metabolome of offspring skeletal muscle following a LPS challenge or commercial Escherichia coli vaccine in utero. Sows were randomly assigned to a treatment beginning at 77 days of gestation: LPS dosed at 2.5 µg/kg bodyweight (LPS; n = 4), LitterGuard-LTC intramuscular vaccine (VAC; n = 3), or saline (CON; n = 3). Gilts from each prenatal treatment were then selected to be necropsied at four timepoints during postnatal life: 3 d (LPS = 6; VAC = 6; CON = 6), 21 d (LPS = 5; VAC = 6; CON = 6), 100 d (LPS = 4; VAC = 5; CON = 6), and 200 d (LPS = 4; VAC = 6; CON = 9). At each time point, gilts were euthanized and a portion of the longissimus dorsi (LD) was snap-frozen in liquid nitrogen. Samples were then homogenized into a powder. Metabolites were extracted by weighing 50 mg of sample into a tube with 1.5 mL of 80% methanol and homogenized using a bead beater. Sample extract was dried with nitrogen gas then derivatized via methoximation and silylation. Untargeted metabolomics was conducted using gas chromatography-mass spectrometry. Mass spectral data were deconvoluted, aligned, and annotated using MS-DIAL. Total ion counts were median normalized and log10 transformed prior to analyses and data were analyzed using MetaboAnalyst by analysis of variance. Significance for all analyses was considered at FDR P = 0.05. Metabolite analysis detected 191 features wherein 105 were annotated via linear retention index and mass spectral match. There were no differences (FDR P > 0.10) in the metabolome of gilts across prenatal treatment or interaction of prenatal treatment × days of age. However, there was an effect of age (FDR P < 0.10) on the metabolomic profile of the LD. There were 63 differentially abundant metabolites across timepoints. Of the differentially abundant metabolites 16 were amino acids or their derivatives (FDR P = 0.045). Five metabolites classified as neurotransmitters were differentially abundant (FDR P < 0.001). Four metabolites with roles in energy metabolism were differentially abundant (FDR P < 0.001). Three fatty acids were differentially abundant (FDR P = 0.043) between timepoints. Finally, there were 6 saccharides which were differentially abundant (FDR P = 0.003) across timepoints. The results of this study imply that prenatal exposure to LPS or a vaccine have no influence on the metabolome of offspring skeletal muscle. However, the metabolome of the LD is subject to changes during postnatal life with alterations in amino acid, fatty acid, and energy related metabolites.