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ARS Home » Pacific West Area » Aberdeen, Idaho » Small Grains and Potato Germplasm Research » Research » Publications at this Location » Publication #45640
Title: SOMATIC EMBRYOGENESIS IN OAT (AVENA SATIVA L.)

Author
item BREGITZER P PHIL - 5366-05-00
item MILACH S K C - UNIVERSITY OF MINNESOTA
item RINES H W - 3640-10-00
item SOMERS D A - UNIVERSITY OF MINNESOTA

Submitted to: Somatic Embryogenesis
Publication Type: Book / Chapter
Publication Acceptance Date: 5/1/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Somatic embryogenesis in oat (Avena Sativa L.) is associated with both nonfriable and friable callus. Embryogenic callus is most routinely initiated by culturing immature embryos of selected genotypes on Murashige and Skoog medium containing 2,4-dichlorophenoxyacetic acid (2,4-D). This callus is a mixture of callus types. Persistent selection during subculturing eventually yields uniformly friable, embryogenic callus. Embryogenic callus induction has been achieved from a number of cultivars by manipulation of 2,4-D and benzylaminopurine (BAP) levels in the culture medium. Friable, embryogenic callus has been successfully used as a source of totipotent target cells for microprojectile bombardment-mediated DNA delivery resulting in production of transgenic tissue cultures that regenerated fertile, transgenic plants.