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ARS Home » Research » Publications at this Location » Publication #47500
Title: PRODUCTION AND IDENTIFICATION OF RECOMBINANT PROTEINS OF SALMONELLA TYPHIMURIUM AND THEIR USE IN DETECTION OF ANTIBODIES IN EXPERIMENTALLY CHALLENGED ANIMALS

Author
item KWANG JIMMY - 5438-01-35
item Littledike, E

Submitted to: Federation of European Microbiological Societies Microbiology Letters
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/24/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: Salmonella infection in cattle is a worldwide problem. Others have evaluated the use of lipopolysaccharides (LPS) for salmonellosis. The diagnostic usefulness of other structures of Salmonella such as flagella (a thread-like structure on the outside of bacteria) has not yet been determined. The flagella proteins are potent antigens, and serological studies on flagella have been used as a basis for classification and identification of Salmonella and other bacteria. Our studies focused on characterizing the nature of the immune response of animals either infected or vaccinated with Salmonella typhimurium. We identified 4 of 15 flagellar proteins, made using genetic engineering techniques, that appeared to be of value for the diagnosis of Salmonella in cattle and sheep.

Technical Abstract: Antibodies to experimental Salmonella typhimurium challenge in cattle and sheep were assessed by 15 recombinant flagellum proteins. The 15 DNA fragments selected for gene expression were derived from external flagellin, hook, hook associated protein, and basal body gene domains. Our efforts were focused on characterizing the humoral immune response of Salmonella infected and vaccinated animals and identifying immunodominant antigenic determinants. This communication reports that the 159-261 a.a. of external flagellum (FliCi-1), 285-331 a.a. of hook protein (FlgE-2), and 140-207 a.a. and 440-537 a.a. of hook associated protein (FlgK-1 and -2) appeared to be the most immune reactive proteins and were recognized by all of the experimental animal sera tested in this study.