PASSIVE IMMUNIZATION OF PIGLETS SUCKLED ON SOWS IMMUNIZED AGAINST CHOLECYSTOKININ.

Authors: Pekas, Jerome; NELSON, DANIEL - MICHIGAN STATE UNIV; GARCIA-SIRERA, J - MICHIGAN STATE UNIV; ROZEBOOM, D - MICHIGAN STATE UNIV; HOGBERG, M - MICHIGAN STATE UNIV

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 6/29/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The objectives were to determine if anticholecystokinin (CCK) immunoglobulins elicited in pregnant sows are secreted by the mammary gland into colostrum and absorbed by suckled piglets. At 63 d gestation 20 gilts were vaccinated with the desulfated C-terminal octapeptide of CCK conjugated to Keyhole limpet hemocyanin (KLH) and 4 control females with KLH alone. Booster vaccinations were on d 77, 91 and 105 of gestation. Essential procedures were described previously. Blood was collected at each vaccination and on d 7, 14 and 21 of lactation. A colostrum sample was collected from each sow and litter size was standardized to 10 piglets. Blood samples were collected from 2 piglets of each litter at d 7 of age. Two treated sows were removed due to poor health. Specific binding of radio iodinated CCK was not demonstrable at 1:10 dilution of serum from control sows. Antisera titers reached values greater than 1:10 in 14 of the remaining 18 treated animals. Mean log titer of sow serum increased to d 7 of lactation and then declined. Mean log titer of colostrum was 2.75, and of piglet serum was 1.99. Log titer of colostrum was highly correlated with log titer of sow serum at booster 3 and at d 7 of lactation. Log titer of piglet serum was highly correlated with log titer of colostrum, and of sow serum at d 7 of lactation. It is concluded that anti-CCK immunoglobulins are transferred from sow to colostrum and absorbed by suckled piglets. Piglet serum titers are similar to those of their dam.