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Title: CHARACTERIZATION OF AVIAN CHLAMYDIA PSITTACI STRAINS USING SEROVAR-SPECIFICMONOCLONAL ANTIBODIES AND RESTRICTION FRAGMENT LENGTH POLYMORPHISM OF THE MOMP GENE

Author
item VANROMPAY, DAISY - UNIV OF GENT, BELGIUM
item ANDERSEN, ARTHUR
item SAYADA, CH. - ROBERT DEBRE HOSP., PARIS
item DUCATELLE, RICHARD - UNIV OF GENT, BELGIUM
item HAESEBROUCK, FREDDY - UNIV OF GENT, BELGIUM

Submitted to: European Committee of the Association of Avian Veterinarians
Publication Type: Abstract Only
Publication Acceptance Date: 3/26/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A panel of five serovar-specific monoclonal antibodies which distinguish the five known avian serovars of Chlamydia psittaci was used to serotype 51 European avian Chlamydia psittaci isolates. Serotyping was performed in an indirect immunofluorescence test. The 51 European isolates included 25 isolates from Psittaciformes, 11 isolates from Columbiformes, 6 isolates sfrom Galliformes, 6 isolates from Passeriformes, and 3 isolates from Anseriformes. All of these were successfully serotyped. One isolate of a duck and two psittacine isolates gave positive immunofluorescence with 2 monoclonal antibodies. These three isolates were cloned using an agar overlay method. Serotyping of the clones demonstrated that each bird was infected with two different serovars. The results show that similar serovars are prevalent in avian species in Europe as in the United States. The results also indicate that birds from a certain order are more susceptible to a distinct serovar. The use of a panel of serovar-specific monoclonal antibodies in the immunofluorescence test provides a reliable method for serotyping avian isolates. Avian C. psittaci strains were also compared by analyzing Alu I restriction patterns of the major outer membrane protein (MOMP) gene after DNA amplification by the polymerase chain reaction. A correlation was found between the results of the serotyping and genotyping.