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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #59704
Title: REGISTRATION OF GIFU B-129-S9 LOTUS JAPONICUS GERMPLASM

Author
item BEUSELINCK, PAUL
item STOUGARRD, J - AARHUS UNIVER, DENMARK

Submitted to: Crop Science
Publication Type: Germplasm Release
Publication Acceptance Date: 7/12/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: This is a Germplasm Release, no Interpretive Summary required.

Technical Abstract: GIFU B-129-S9 Lotus japonicus (Regel) Larsen germplasm was released by the Agricultural Research Service-USDA in cooperation with the Department of Molecular Biology, Aarhus University, Denmark in April 1995. GIFU B-129- S9 is suitable for genetic and molecular biology research because i) it is a diploid (2n=2x=12), perennial, autogamous legume with good seed set, and a sexual regeneration time from of approximately 3 months, ii) has a haploid genome size estimated to be relatively small at 0.5 pg per haploid complement, and iii) is susceptible to Agrobacterium tumefaciens and transgenic plants can be regenerated after hygromycin, geneticine, or kanamycin selection. Stable transformation followed by regeneration has been demonstrated. Transformed plants can be generated efficiently and quickly using GIFU B-129-S9 germplasm. Flowering is indeterminate. Plants are cross-fertile making classical genetic studies possible. Fast- (Rhizobium loti) and some slow-growing (Bradyrhizobium sp.) strains of rhizobia will nodulate GIFU B-129-S9 germplasm, but R. loti strain NZP 2235 is recommended for fast and effective nodulation.