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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Publications at this Location » Publication #60328

Title: PHYSICAL AND GENETIC MAPPING OF MAIZE CHROMOSOME 9S USING MUTATIONS WITH TERMINAL DEFICIENCIES

Author
item CHAO, SHIAOMAN - UNIV OF MO
item GARDINER, JACK - UNIV OF IA
item COE JR, EDWARD

Submitted to: Food and Agriculture Organization International Atomic Energy Agency
Publication Type: Abstract Only
Publication Acceptance Date: 6/18/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Using chromosome materials developed by Dr. Barbara McClintock to break chromosomes and produce deletions of ends at a series of points, answers to two questions have been sought. The first question is whether the genetic-map order of genes and of molecular markers in the short arm of chromosome 9 of corn is confirmed by the exact criterion of the physical order of deletions; the data confirm that the order measured by both means is the same. The second question is whether the molecular size and the DNA in end portions of a newly broken chromosome can be distinguished from unbroken ends; the data are explicitly clear in showing distinguishable end segments after breakage, and they further identify an end marker for the normal end portion of the chromosome map. The value of this specific information is basic, but the generalizable implications strengthen confidence in map development for the many applied purposes for which mapping is used.