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Title: CHARACTERIZATION OF GENES DIFFERENTIALLY EXPRESSED AMONG GREENBUG- SUSCEPTIBLE AND -RESISTANT LINES OF WHEAT

Author
item CUSHMAN, MARY - OKLAHOMA STATE UNIV
item GUENZI, AARON - OKLAHOMA STATE UNIV
item Veal, Rita
item Porter, David
item SMITH, ED - OKLAHOMA STATE UNIV

Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/29/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Damage to wheat by greenbug (Schizaphis graminum Rondani) constitutes an economically significant biotic stress in western wheat growing states. Wheat germplasm GRS1201, carrying a translocated rye chromosome arm (1RS) from Insave rye, is resistant to four major greenbug biotypes: B, C, E, and G. However, linkage of these gene(s) to unfavorable alleles for dough quality confounds their deployment, and the lack of recombination between wheat and rye chromosomes makes introgression of these gene(s) into a desirable wheat cultivar difficult. Our goal is to isolate constitutively expressed genes from GRS1201 and Insave rye that are not expressed in greenbug-susceptible wheat parents of GRS1201 using Differential Display Reverse Transcription PCR (DDRT-PCR). To date, Northern analysis has confirmed one differential product specific to GRS1201, Insave and TAM105/Amigo, each carrying 1RS. Candidate genes will be sequenced and database searches will identify potential homology to known genes. Future experiments will demonstrate whether these genes map to 1RS. Ultimately transformation experiments may confirm their role in resistance to greenbug infestation.