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Submitted to: Journal of Liquid Chromatography
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 1/25/1996 Publication Date: N/A Citation: N/A Interpretive Summary: This article describes a high-performance liquid chromatographic (HPLC) method for measuring cuscohygrine in coca leaves. A reliable HPLC method is needed for the analytic separation of platnt alkaloids that can not be separated by gas chromatography. This new methodology will be disseminated to researchers in the scientific community and analysts who are conducting similar plant research at state and federal agencies such as Drug Enforcement Administration Special Testing and Research Laboratory, Mclean, VA. The separation of cuscohygrine was accomplished with a weak cation exchange column. The HPLC method, which was tested and validated, will provide analysts with a sensitive, simple and rapid method for determining cuscohygrine in plant extracts. Technical Abstract: A novel high-performance liquid chromatographic (HPLC) method is described for the determination of the alkaloid cuscohygrine (1,3-bis(1-methyl-2-pyrrolidinyl)-2-propanone) in air-dried leaves of Erythroxylum coca var coca. The analysis was performed on a weak cation exchange HPLC column using a mobile phase consisting of MeOH: 0.05 M KH2HPO4, pH 7 (75: 25%, v/v) and a UV detector set at 220 nm. Cuscohygrine content in E. coca leaves was determined as 0.21 +/- 0.02% (C.V. = 6.26%) using an improved extraction procedure for cuscohygrine. |
