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Title: NMR STUDIES OF THE ZINC FINGER MOTIFS OF URBS1 PROTEIN

Author
item ZHAO, QIN - UNIVERSITY OF WISCONSIN
item AN, ZHIQIANG - UNIVERSITY OF WISCONSIN
item Leong, Sally
item MARKLEY, JOHN - UNIVERSITY OF WISCONSIN

Submitted to: Frontiers of Nucleur Magnetic Resonance in Molecular Biology
Publication Type: Abstract Only
Publication Acceptance Date: 4/2/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The goal of our research is to elucidate the structure and function of the zinc-finger like motifs of Urbs1 using multidimensional NMR spectroscopy. Urbs1 is a putative transcription repressor of the sidl gene which encodes an enzyme involved in ferrichome siderophore biosynthesis in the fungus Ustilago maydis. The Urbs1 protein itself has a molecular weight of 100 kD and contains two zinc-finger-like motifs (GATA- 1 type). Expression or structural studies of this protein and its motifs have not been reported. An 8 kD polypeptide encompassing the C-terminal finger domain of the urbs1 protein has been subcloned and overexpressed in E.coli using the pET system (Novagen). Preliminary NMR results will be described.